强啡肽对实验性神经细胞老化过程中M胆碱能受体位点数影响的研究

目的　探讨强啡肽 (DYN)对实验性神经细胞 M受体位点数的影响。方法　采用小鼠神经母细胞瘤细胞 (NBA2 )无血清培养建立神经细胞老化实验研究模型。以 M受体放射性配基分析术研究 DYN对实验性神经细胞的 M受体位点数的影响。结果　两种不同浓度的 DYN皆可使M受体位点数下降 (P<0 .0 1 ) ,以高浓度组更显著。结论　 DYN使 M受体位点数下降的作用可能是延缓实验性神经细胞老化的机制之一。(最终浓度为 0 .1nmol/L) ,以测定非特异性结合。各管加入2 0 0 μl膜蛋白液 ,37℃振荡温育 4 0 min。冰冷缓冲液终止反应。6 9型纤维滤膜三层抽滤并淋洗 ,滤膜烘干 ,投入 5 ml闪烁液(含 0 .4 % PPO的三甲苯 )中 ,以 YSL- 76型液闪计数器做固相测定。1.8　 数据处理　实验得到特异性结合的技术率 ,被测定效率除 ,等于特异性结合衰变率 ,再除以放射性配基活度 ,即等于特异性结合位点数〔7〕。采用 POMS方差分析 ,经 IBM微机处理数据。2　结果与讨论DYN对 NBA2 细胞 M胆碱能受体位点数的影响 ,见表 1。表 1　 DYN对 M受体位点数的影响 (nmol/10 6 细胞 )组别 n M受体位点数 (x± s)BSD组 5 449.796± 356.730AID组 5 486.941± 2 4 7.1 65DYN- 7组 5 1 4 5.71 4± 77.82 61 )DYN- 8组 5 1 51 .873± 95.80 61 )　　注 :DY

The effect of dynorphin on muscarine cholinergic receptors during experimental neuronal aging

Objective To observe the effects of dynorphin (DYN) on muscarine (M) cholinergic receptors of experimental neuron. Methods Free-culture of mouse neuroblastoma A2 (NBA2) cells was used to make the experimental model for the study of neuronal aging. The values of M-cholinergic receptors were measured. Results After addition of DYN with two different concentrations into the culture media, the values of M-cholinergic receptors decreased in DYN-7 and DYN-8 groups (P<0.01), significantly in high concentration group. Conclusions The results suggest that the decrease of M-cholinergic receptors induced by DYN may play an important role in the modulation of experimental neuronal aging.