侧脑室注射芹菜素对情期雌性大鼠生殖激素含量影响的研究

目的 探讨芹菜素在生殖轴调控中的作用及作用部位。方法 通过阴道涂片选用情期成年雌性大鼠50只，随机分为五组，分别侧脑室注入人工脑脊液、二甲基亚砜、芹菜素10^-5M、hCG及芹菜素10^-5M＋hCG，各组再分为3d组、5d组。分别于侧脑室注射后的3d、5d断头取血、卵巢及垂体，应用放射免疫方法分别测定血清、卵巢组织及垂体组织中FSH、LH、E2及P含量，以观察浓度为10^-5M的芹菜素对生殖轴的作用。结果 1．人工脑脊液组与二甲基亚砜组无明显差异。2．单用100^-5芹菜素组与人工脑脊液组相比FSH、LH、E2、P的含量数值小，其差异有显著性。3．单用hCG组与人工脑脊液组相比FSH、LH的含量数值小，而E2、P的含量数值大，其差异均有显著性。4．10^-5M M芹菜素与hCG合用组与单用10^-5M芹菜素组相比FSH及LH的含量数值小，而E2、P的含量数值大，其差异均有显著性。5．10^-5M芹菜素与hCG合用组与单用hCG组相比FSH、LH的含量数值大，而E2、P的含量数值小，其差异均有显著性。结论 芹菜素在下丘脑水平发挥作用，进而作用于腺垂体、卵巢。10^-5M芹菜素能够抑制FSH、LH的合成，从而抑制E2、P的分泌。10^-5M芹菜素与hCG合用时可表现出抗雌激素的作用。

The Research on the Effect of Apigenin Injected to Lateral Brain Ventricle on the Reproductive Axis of Female Rat

Objective The main idea of this study was to investigate the effect and the position of apigenin on the reproductive axis of female rat in vitro. Methods Adult female S-D rats were injected ACSF,DMSO,10~(-5)mol/L Api,10~(-5)mol/L Api +hCG,through lateral brain ventricle and decapitated to take blood,pituitary and ovary.FSH,LH,E_2 and substance P in serum,pituitary and ovary were determined by radiommunassay. Results(1)The ACSF group wasn't significant deviation than the DMSO group.(2)FSH,LH,E_2 and P of the Api group were significance level than the ACSF group.(3)FSH,LH of the DMSO group were significant decrease than the ACSF group,but E_2,P were significant figures.(4)FSH,LH of the Api+hCG were significant decrease than.the Api group,but E_2,P were significant figures.(5)FSH,LH of the Api+hCG were significant figures than the hCG group,but E_2,P were significant level. Conclusions Apigenin inhibits FSH,LH,E_2 and substance P release in the level of hypothalamus.10~(-5)mol/L Api+hCG have beneficial for morphology of hormone.