东方田鼠乳酸脱氢酶同工酶的研究

为了解普通级KM、NIH、BABL/c小鼠群的健康状况,对2288只淘汰的繁殖群小鼠进行剖检。结果发现KM繁殖群1（KM-1）、KM繁殖群2（KM-2）、NIH、BALB/c小鼠的肺部病变分别为：19.20%（192/1000）、11.85%(34/287)、7.80%(39/500)、11.98%(60/501);其中肺炎分别占：13.3%()133/1000)、5.57%（16/287）、4.60%(23/500)、9.38%(47/501);肺肿瘤分别占：5.90%(59/1000)、6.27(18/287)、3.20%(16/500)、2.59%(13/501)。肝脏病变分别为：20.7%(207/1000)、5.57%(16/287)、4.60%(23/500)、1.80%(9/501)。脾脏病变分别为：18.0%(18/1000)、4.88%(14/287)、2.20%(11/500)、1.20%(6/501)。其它器官系统也出现程度不同的病变。剖检结果提示：普通级小鼠群必须提高级别,从实验动物设施、饲养环境条件、饲养方法等诸多方面达到清洁极标准,否则普通级小鼠将影响科研、生产、鉴定等试验结果的准确性、可靠性及重复性。

Study on Lactate Dehydrogenase Isozyme of Microtus fortis

Objective To study the distribution of the lactate dehydrogenase isoenzyme in different tissues of field voles ( Microtus fortis ). Methods The samples were studied by polyacrylamide gel electrophoresis (PAGE) and stained by sodium lactate. Results There was only LDH 5 in plasma of field voles from all three regions and in sera of field voles from Dongting region and from Ningxia Qingtongxia region. There were two kinds of LDH isoenzymes(LDH 1 and LDH 5)in sera of field voles from Dongbei region. Five kinds of LDH isoenzymes were found in kidney and lung of field voles from three regions and in liver of field voles from Dongbei region, but LDH 5 was dominated in liver of the two other field voles(more than 95%). Conclusion LDH isoenzymes of field voles from Dongbei region were different to some extent from the two other regions.