波形蛋白在不同类型慢性鼻-鼻窦炎中的表达及意义

目的:探讨波形蛋白(VM)在不同类型的慢性鼻-鼻窦炎中的表达和分布情况及意义。方法:将慢性鼻-鼻窦炎患者分为嗜酸粒细胞性鼻息肉(10例)、非嗜酸粒细胞性鼻息肉(12例)和不伴息肉的单纯慢性鼻-鼻窦炎(10例),将鼻中隔偏曲的肥大下鼻甲(10例)作为对照组。用免疫组织化学染色检测各组中VM的表达。并运用免疫荧光双染检测VM阳性的细胞是否同时表达上皮的连接标记上皮性钙黏蛋白,用来鉴别VM在上皮中的表达是否属于上皮来源的细胞。结果:伴和不伴息肉的慢性鼻-鼻窦炎各组以及对照组的上皮与间质中均出现VM的阳性表达。但VM阳性的细胞主要分布于黏膜的间质包括成纤维细胞、血管内皮细胞和其他间充质细胞。伴息肉的慢性鼻-鼻窦炎组VM的表达较其他组下调(P<0.05)。VM阳性的细胞为上皮来源的细胞,上皮层的VM阳性细胞尤其见于各种类型的鼻-鼻窦炎。结论:VM不仅分布于鼻黏膜的间质而且分布于黏膜上皮,这一现象尤其见于慢性鼻-鼻窦炎,且这些VM阳性的细胞不是白细胞,可能是上皮-间充质细胞转分化的结果。VM与鼻-鼻窦炎的组织学特征密切相关,可能在慢性鼻-鼻窦炎的发病过程中发挥重要的作用。

Expression and significance of vimentin in different types of chronic rhinosinusitis

Objective: To explore the expression and distribution of vimentin in different types of chronic rhinosinusitis and its significance.Method: There were four groups including control(10 samples),Eos CRSwNP(10 samples),non-Eos CRSwNP(12 samples) and CRSsNP(10 samples).The expression of vimentin in chronic rhinosinusitis were detected by immunohistochemistry technique.The double-immunofluorescence was used to detect the positive staining of both vimentin and E-cadherin,both of which were the marker of epithelial cells.Result: The positive staining of vimentin were observed both in epithelium and lamina propria.The expression of vimentin were found in myofibroblast,endothelium and other mesenchymal cells.The vimentin positive cells in epithelium were epithelial cells but not mesenchymal cells,as they also expressed E-cadherin.Conclusion: The vimentin positive staining cells distribute in lamina propria and epithelium of both normal nasal mucosa and chronic rhinosinusitis.The positive staining epithelial cells may generate from epithelial-mesenchymal transition.So the vimentin may play an important role in the development of chronic rhinosinusitis.