O139群霍乱弧菌L型变异和诱导实验的观察及致病性的探讨

[目的]探讨O139群霍乱弧菌L型变异及其与ElTor型的R型血清的关系。[方法]2002～2005年,我们采用酚肉汤传代、抗生素、胆盐和蒸馏水等实验室诱导方法进行观察。[结果]发现O139群霍乱弧菌6菌株经上述方法诱导可发生L型变异,其菌落特征和菌体形态均与L型的描述相符。[结论]实验结果证实O139群霍乱弧菌可诱导成L型,通过与O1群的R型血清凝集状况,表明其L型菌株未见到抗原的完全消失,其变异过程与ElTor弧菌相似,关系密切,推测自然界中亦存在O139群霍乱弧菌L型,且仍具有致病性。

Laboratory Research on L-form Variation and Pathogenicity of Vibrio Cholerae O139

[Objective]To discuss the Variation of Vibrio cholerae O139 L-forms anf the correlation between the L form and R-form sernm.[Methods]To observe the variation of Vibrio cholerae O139 L-forms with induction of antibiocs,sodium deoxycholate and distilled water.[Results]L-forms of Vibrio cholerae O139 ,whose characteristics of colonies and morphology of bacteria were accorded to L-forms,may be induced by antibiocs,sodium deoxycholate and distilled water.[Conclusion]Vibrio cholerae O139 L-forms can be induced. The variable procedure is similar to Vibrio cholerae El Tor.It is possible that there exist L-forms of Vibrio cholerae O139 in natural environment.