不同负载时间下红细胞内海藻糖和葡萄糖含量比较

目的探索适合海藻糖和葡萄糖联合负载红细胞的时间,为冷冻干燥红细胞提供基础。方法分别采用0、0.125、0.25、0.5和1 mol/L的海藻糖联合葡萄糖,在37℃下负载红细胞4、6和8 h。然后检测负载红细胞内海藻糖和葡萄糖的浓度。结果随着负载时间的延长,进入红细胞内的海藻糖和葡萄糖数量增加。并且随着负载液浓度的增加,红细胞内的海藻糖和葡萄糖数量增加。负载时间为8 h时,进入红细胞的葡萄糖比6 h组明显增加。但是,延长负载时间为8 h后,进入红细胞的海藻糖浓度与6 h组比较,差异无统计学意义。结论海藻糖联合葡萄糖负载红细胞6 h后,进入红细胞内的海藻糖和葡萄糖,能够满足冰冻干燥红细胞的负载要求。

The optimum time of trehalose and glucose loading red blood cells for lyophilization

Objective The aim of this study is to explore the optimum time of trehalose and glucose loading red blood cells for lyophilization.Methods Red blood cells were loaded with 0,0.125,0.25,0.5 or 1 mol/L trehalose and glucose at 37 ℃ for 4,6 or 8 hours,followed by determination of the levels of trelose and glucose in the loaded red blood cells.Results The concentrations of trelose and glucose in the loaded red blood cells were increased in a time-and dose-dependent manner.The level of glucose in the loaded red blood cells was significantly higher in the 8h than in the 6h group,but that of trehalose showed no significant difference between the two.Conclusion The concentrations of trehalose and glucose with which red blood cells were loaded for 6 hours could satisfy the requirement of lyphilization.