| HPLC法测定肾乐胶囊中橙皮苷的含量 |
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| 引用本文： | 白林,郭兴道,任.HPLC法测定肾乐胶囊中橙皮苷的含量[J].中国药物应用与监测,2012,9(3):139-141. |
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| 作者姓名： | 白林 郭兴道 任 |
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| 作者单位： | 1. 解放军总医院药品保障中心药检室,北京,100853
2. 河北北方学院,河北张家口,075000 |
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| 摘 要： | 目的:建立HPLC测定肾乐胶囊中橙皮苷含量的方法。方法:采用HPLC法对肾乐胶囊中橙皮苷进行含量测定。测定条件:采用Diamonsil C18色谱柱(250 mm×4.6 mm,5μm),流动相为甲醇-水(40∶60),检测波长:283 nm,流速:1.0mL.min-1,柱温:35℃。结果:在本法条件下,橙皮苷与其他杂质峰分离良好,进样量在8.544～85.44 mg.mL-1与峰面积呈良好的线性关系(r=0.999 9),平均加样回收率为101.6%,RSD为0.54%(n=6)。三个批次的肾乐胶囊中橙皮苷的平均含量为2.75 mg.g-1。结论:采用该方法测定肾乐胶囊中的橙皮苷,简便易行,结果准确,可作为肾乐胶囊的质量控制方法。
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| 关 键 词： | 高效液相色谱法 肾乐胶囊 橙皮苷 |
Content determination of hesperidin in Shenle capsule by HPLC |
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| Authors: | BAI Lin GUO Xing-dao REN Wei |
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| Institution: | 1. Department of Pharmaceutical Care, PLA General Hospital, Beijing 100853, China; 2. Hebei North University, Zhangjiakou 075000, China) |
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| Abstract: | Objective： To determine the content of hesperidin in Shenle capsule by HPLC. Methods： An HPLC method was used with Diamonsil C18 column （250 mm×4.6 mm, 5μm） and the mobile phase consisted of methanol-water （40 ： 60）. The flow rate was 1.0 mL-min-1, the detection wavelength was 283 nm and column temperature was 35℃. Results： The calibration curve was linear in the range of 8.544 - 85.44 mg-mL1 （r = 0.999 9）. The average recovery was 101.6% and the RSD was 0.54% （n = 6）. The average content of hesperidin in Shenle capsule was 2.75 mg. g-1. Conclusion： This method is accurate and feasible, and it can be applied as the quantitative method of hesperidin in Shenle capsule. |
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| Keywords: | HPLC Shenle capsule Hesperidin |
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