血三七水煎液对大鼠局灶性脑缺血再灌注损伤的保护作用

目的研究血三七Polygonum amplexicaule D.Don var.sinense Forb.et Hemsl.ex Stew.水煎液对大鼠脑缺血再灌注损伤的保护作用,并探讨其机制。方法 180只雄性SD大鼠随机分为脑缺血再灌注（模型）组,假手术组,银杏叶提取物（0.2g/kg）组,血三七水煎液高、中、低剂量（0.3、0.2、0.1 g/kg）组,给药组术前连续ig 14 d,于末次给药60 min后行手术,采用结扎颈外动脉建立大鼠大脑中动脉局灶性脑缺血再灌注损伤模型,观察血三七水煎液组对脑缺血再灌注损伤大鼠神经功能、脑梗死组织质量比、脑含水量、脑组织中生化指标的影响。结果与模型组比较,血三七水煎液高、中剂量组,银杏叶提取物组均可明显改善神经功能障碍,明显降低脑梗死组织质量比、减低脑组织中含水量,升高大鼠脑组织中SOD活性,降低MDA、NO的量（P〈0.05、0.01）。结论血三七水煎液对大鼠脑缺血再灌注损伤具有保护作用,其作用机制可能与其抗自由基和抑制NO生成有关。

Protection of Polygonum amplexicaule var. sinense water decoction on focal cerebral ischemia-reperfusion injury in rats

Objective To observe the protective effect of Polygonum amplexicaule var. sinense water decoction（PAWD） on focal cerebral ischemia-reperfusion（I/R） injury in rats, and to explore its mechanisms. Methods SD male rats（120 rats） were randomly divided into I/R, Sham, Ginkgo biloba extract（GBE, 0.2 g/kg）, high-, mid-, and low-dose（0.3, 0.2, and 0.1 g/kg） PAWD groups. The animals were ig administered with drugs for 14 d. The operation was performed to induce the middle cerebral artery occlusion model in rats with the method of intraluminal thread at 60 min after the last administration via ligation of external carotid artery. After ischemia for 2 h and repefusion for 24 h in each model rat, the influences of PAWD on nerve function, tissue weight rate of cerebral infarction, water contents in brain, and biochemical indicators in brain were determined. Results Compared with I/R group, high-, mid-dose PAWD, and GBE could obviously improve the neurological dysfunction of rats, reduce the brain water contents, decrease tissue weight rate of cerebral infarction, increase the activities of SOD, and. reduce MDA and NO contents（P〈0.05 or 0.01）. Conclusion PAWD has protective effect on cerebral I/R injury, and the mechanism may be related to attenuating free radical and NO.