大孔吸附树脂纯化两色金鸡菊总黄酮的工艺研究

目的研究大孔树脂纯化两色金鸡菊总黄酮的工艺条件及参数。方法采用静态吸附解吸法优选适宜的大孔树脂,以树脂吸附量、总黄酮解吸率为考察指标,对筛选出的树脂进行工艺优化。结果 D-101树脂具有较好的吸附及解吸附性能,其最佳工艺为：上样液质量浓度3.4 mg/mL,树脂的上样量为15 mL/g,上样液pH值为5.0,上样流速1.0 mL/min,6 BV去离子水洗脱除杂后,用pH 5.0的70%乙醇以1.5 mL/min洗脱5 BV;所得两色金鸡菊总黄酮质量分数为73.5%,得率为8.8%。结论 D-101树脂综合性能好,适合于两色金鸡菊总黄酮的分离纯化。

Purification of total flavonoids from Coreopsis tinctoria with macroporous resins

Objective To explore the technological parameters and the purification process of total flavonoids from Coreopsis tinctoria. Methods Nine types of macroporous resins were compared through static adsorption-desorption methods with adsorbability and desorption ratio as indexes to optimize the purification parameters. Results D-101 resin showed better adsorption and desorption parameters, and the optimal parameters for purification process were as follows： the concentration of sample extract was 3.4 mg/mL, the loaded amount was 15 mL/g, pH value was 5.0, the flow rate was 1.0 mL/min, and after being eluted with 6 BV water, the optimal solution for desorption was 70% ethanol in pH 5.0 with 5 BV at the flow rate of 1.5 mL/min. The purity of total flavonoids was 73.5%, and the yield was 8.8%. Conclusion D-101 resin shows significant effect on the purification of flavonoids which is feasible to separate and purify total flavonoids from C. tinctoria.