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| 白花蛇舌草多糖的分离纯化及PMP柱前衍生高效液相分析 | |
| 引用本文： | 钱韵旭,刘裴,李莉,李晓蕾. 白花蛇舌草多糖的分离纯化及PMP柱前衍生高效液相分析[J]. 云南中医学院学报, 2010, 33(3): 43-46 |
| 作者姓名： | 钱韵旭 刘裴 李莉 李晓蕾 |
| 作者单位： | 1. 昆明理工大学,云南昆明,650224 2. 云南中医学院,云南昆明,650500 3. 昆明医学院,云南昆明,650500 |
| 摘 要： | 经热水提取、醇沉、大孔树脂分离、三氯乙酸脱蛋白、H2O2脱色、透析、DEAE-52纤维素和Sephadex G-200凝胶柱层析，建立了白花蛇舌草多糖的分离纯化方法。制备的ODI经紫外扫描及HPGPC测试，证实为单一纯品。ODI经PMP柱前衍生高效液相法测定，确定其由鼠李糖、葡萄糖、半乳糖、木糖和阿拉伯糖组成。 |
| 关 键 词： | 白花蛇舌草 多糖 单糖 高效液相 1-苯基-3甲基-5-吡唑啉酮 |
Isolation and Purification of Polysaccharide from Hedyotis Difusa Wilid and Analysis on its Composition by Pre-column Derivatization HPLC |
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| QIAN Yun-xu,LIU Pei,LI Li,LI Xiao-lei. Isolation and Purification of Polysaccharide from Hedyotis Difusa Wilid and Analysis on its Composition by Pre-column Derivatization HPLC[J]. Journal of Yunnan College of Traditional Chinese Medicine, 2010, 33(3): 43-46 | |
| Authors: | QIAN Yun-xu LIU Pei LI Li LI Xiao-lei |
| Affiliation: | 1. Kunming University of Science and Technology, Kunming Yunnan 650224, China; 2. Yunnan University of TCM, Kunming Yunnan 650500, China; 3. Kunming Medical University, Kunming Yunnan 650500, China) |
| Abstract: | A method of preparation of polysaccharide from Oldenlandia diffusa wild was identified, using hot water extract method, ethanol precipitation, removing the proteins by TCA reagent, decoloring with hydrogen peroxide and dialyzing by distilled water. After that, one of polysaccharides was isolated and purified through DEAE -52 and SephadexG -200 chromatography, which was named OD I. The purity of OD I was examined by UV and HPCPC. The composition of monosaccharides of OD I was mannose, glucose, galactose, xylose and arabinose which was determined by HPLC with pre -column derivatization. |
| Keywords: | oldenlandia diffusa wild polysaccharide monosaccharide high performance liquid chromatography PMP |
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