VEGF-C在非小细胞肺癌中的表达及其临床意义

目的分析血管内皮生长因子C(VEGF-C)与肿瘤TNM分期、病理类型、分化程度的关系,探讨其与非小细胞肺癌(NSCLC)侵袭、转移和预后的关系。方法选取胸外科2004～2008年手术切除的60例NSCLC患者的标本(其中鳞癌26例,腺癌34例),并同期选取15例正常支气管肺黏膜组织作为对照,所有标本均切片做HE染色,显微镜下作出病理诊断。运用免疫组织化学链霉素亲生物素-过氧化物酶法(S-P法)研究肿瘤组织、正常肺组织中VEGF-C的表达情况,显微镜下计数阳性细胞数,并进行统计学分析。结果 VEGF-C在NSCLC中存在较高表达,与患者的年龄、临床分期、分化程度及淋巴结转移关系密切,而与患者的性别、肿瘤组织学类别无关。结论 NSCLC组织中VEGF-C高表达,提示恶性度高,易发生淋巴结转移。其有助于评估肺癌的转移趋向,分析病期,指导术后治疗方案。

Expression and Clinical Significance of VEGF-C in Non-small Cell Lung Cancer

Objective To study the relationship between the expression of VEGF-C and the TNM staging,pathological type and differential degree in non-small cell lung cancer(NSCLC).Meanwhile,to explore its relationship with invasion,metastasis and prognosis of NSCLC.Methods Sixty cases(26 cases of squamous cell carcinoma,34 cases of adenocarcinoma) of NSCLC who received resection during 2004 and 2008 were selected and 15 cases of normal bronchopulmonary mucosa were selected as control.All the samples were stained by HE method to confirm their pathological characters under microscope.VEGF-C expression of the tumor tissue and normal tissue was studied by immunohistochemistry S-P method,positive cell number was counted under microsope,and statistical analysis was done.Results It was found that the positive expression rates of VEGF-C was significantly higher in NSCLC than those in normal lung tissues.The expression of VEGF-C were closely associated with patients' age,pathologic grade,TNM-stage and lymph node metastasis,but not related to the patient gender and tumor histological category.Conclusion VEGF-C overexpression in NSCLC indicates high malignancy,which is prone to lymph node metastasis.It' s helpful to assess the metastasis trend,analyze the pathological stage and guide the postoperative treatment.