不同穴位针刺对免疫抑制大鼠T细胞极化及相关因子的影响

目的:观察不同穴位针刺后对免疫抑制模型大鼠T淋巴细胞亚群及外周血IFN-r、IL-4含量的影响,探讨不同穴位针刺后对免疫抑制大鼠T细胞极化及相关细胞因子的影响。方法:选取健康SD雄性大鼠45只。随机分5组,正常对照组(A组),模型对照组(B组),模型加针刺头针组(C组),模型加针刺体针组(D组),模型加针刺头针体针组(E组)。采用环磷酰胺注射法制备免疫抑制模型,分别选取"头穴(百会及旁开4 mm两针丛刺)"、"体穴(足三里和关元)"、"头穴加体穴"进行针刺,时间为20 min,每隔5 min进行补法捻针。采用ELISA法测定血清IFN-r和IL-4含量;采用流式细胞仪法测定外周血CD4+TC和CD8+TC含量。结果:B组大鼠外周血CD4+TC含量显著下降,与A组比较有非常显著性差异(P0.01);经针刺治疗后,针刺各组外周血CD4+TC显著升高,与B组比较有显著性差异(P0.05);治疗前后各组CD8+TC含量无显著变化(P0.05)。治疗前B组IFN-r含量下降、IL-4含量升高,与正常A组比较有显著性差异(P0.05),治疗后各组IFN-r含量显著升高,与B组比较均有显著性差异(P0.05),各组间以E组疗效最佳,效果优于其它治疗组。结论:头针加体针的配穴方式可显著提高免疫抑制大鼠血中CD4+T淋巴细胞的含量,促使TH细胞向Th1型转化,增强机体细胞免疫的功能,疗效优于体针和头针组。

The Effect of Different Acupuncture on the T-cell Polarization and Related Factors in Rats with Immune Suppression

Objective: To observe the peripheral blood T lymphocyte subsets and IFN-r,IL-4 levels of immunosuppressive rats after different acupuncture points,and to explore the effect of different acupuncture points on the immunosuppressive rats.Methods:45 healthy SD rats were randomly divided into normal control group(A group),model control group(B group),the model plus head acupuncture group(C group),the model plus body acupuncture group(D group),the model plus head acupuncture and body acupuncture group(E group).Im...