紫草素抑制胃癌细胞增殖、黏附及诱导凋亡

目的探讨异紫草素AJ-7及其衍生物AJ-9、AJ-5和AJ-8对人胃癌细胞SGC 7901增殖及黏附的抑制作用,以及凋亡诱导作用。方法应用MTT比色法检测新疆紫草素对细胞的增殖、黏附抑制作用,用流式细胞术(FCM)、Annexin-V-FITC联合法及透射电镜检测新疆紫草素对胃癌细胞的凋亡诱导效应。结果新疆紫草素能够抑制胃癌细胞的增殖,并呈浓度时间依赖性。FCM出现明显的亚二倍体峰并将细胞阻滞于G1期,Annexin-V-FITC联合法染色可见细胞凋亡的特征性贯穿胞质穿过胞膜的红色颗粒,电镜下可见染色质边集及生发小体的凋亡形态特征。结论新疆紫草素能够有效抑制胃癌细胞的增殖、黏附,并诱导凋亡。

Study of alkannin inhibiting the proliferation, adhesion and inducing apoptosis of the gastric cancer cell line

AIM To investigate the effect of iso-alkannin AJ7 and its derivatives AJ-9,AJ-5 and AJ-8 inhibiting the proliferation,adhesion and inducing apoptosis of gastric cancer cell line SGC 7901.METHODS The changes of the proliferation and adhesion of the alkannin to the cells were measured by methyl thiazolyl tetrazolium(MTT).The effect of inducing apoptosis of the alkannin to the cells were observed by the flow cytometry(FCM),Annexin-V-FITC staining and transmission electron microscope.RESULTS The alkannin could inhibit the proliferation of the cells with dependence of concentration and time.FCM appeared obvious inferior diploid peak and blocked the cells to the G_1 phase.The red particles throughout the cytoplasm and permeated the plasma membrane could also be seen.Chromatin lining and breeding body could be seen under the transmission electron microscope.CONCLUSION The iso-alkannin and its derivatives could effectively inhibit the proliferation and adhesion of the gastric cancer cells and induced apoptosis.