组织扩张与机械牵拉对皮肤组织表达硫氧还蛋白影响的初步研究

目的 探讨硫氧还蛋白(TXN)在扩张皮肤组织与正常皮肤组织中的表达差异.方法 选取近两年在我院行额部扩张器取出术的患者13例,收集患者额部扩张器张力最大点(扩张组)以及颞部行附加切口处(对照组)的全层皮肤.免疫组织化学染色、Western blot检测TXN蛋白在扩张皮肤组织及正常皮肤组织中的表达.结果 免疫组织化学检测发现,扩张皮肤组织中TXN阳性细胞明显多于正常皮肤组织(P＜0.05)；Western blot检测发现,扩张皮肤组织中TXN表达高于正常皮肤组织.结论 扩张皮肤组织中TXN蛋白表达高于正常皮肤组织,为进一步研究TXN在组织扩张中的作用提供理论基础.

The Differential Expression of TXN between Expanded Skin Tissue and Normal Skin Tissue in Vivo

Objective To investigate the difference of TXN protein expression between the expanded skin and the normal skin. Methods From 2010 to 2012, 13 patients underwent forehead expanded flap transfer were included in this study. Full-thickness skin were harvested from central forehead skin with maximum strain （expanded group） and temporal additional excision sites （control group）, hnmunohistochemistry and western blot were used to detect the TXN expression between the expanded skin and the normal skin. Results The positive cell number of TXN in the expanded skin tissue was more than that in normal skin tissue showed by immunohistoehemistry staining. Western bolt showed that the TXN expression in the expanded skin tissue is higher than in normal skin tissue. Conclusion The TXN protein expression in expanded skin tissue is higher compared with normal skin tissue. It provides the theoretical basis for the further research of TXN function on skin tissue expansion.