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低强度微波辐射对大鼠神经细胞死亡率影响的实验研究
引用本文:刘伟国,杨小锋,高峰,姜秀意,童建忻. 低强度微波辐射对大鼠神经细胞死亡率影响的实验研究[J]. 浙江创伤外科, 2001, 6(3): 141-142
作者姓名:刘伟国  杨小锋  高峰  姜秀意  童建忻
作者单位:1. 杭州,浙江大学医学院附属第二医院 310009
2. 浙江大学医学院附属第二医院 310009
3. 浙江大学医学院
4. 美国华盛顿大学医学院
基金项目:本课题为浙江省科委基金资助项目(资助号: 991106449)
摘    要:目的 探讨低强度微波辐射对大鼠神经细胞死亡率的影响。方法 在对体外新生大鼠(0~1天)皮层神经细胞进行原代培养的基础上,以频率900MHz,功率密度分别为0.025mW/cm^2、0.05mW/cm^2、0.1mW/cm^2的低强度微波辐射4、8、12、16、20、24小时。检测细胞死亡率。结果 和对照组相比,0.05mW/cm^2功率密度组辐射12小时、0.1mW/cm^2功率密度组辐射8小时后均可引起神经细胞细胞死亡率增高,且随着辐射时间的延长,细胞死亡率也逐渐上升,0.025mW/cm^2功率密度组未见明显改变。结论 长期连续的低强度微波辐射对培养大鼠神经细胞存在损伤作用。

关 键 词:低强度 大鼠 神经细胞死亡 实验研究 微波辐射 皮层神经细胞 对照组 影响 体外 改变
修稿时间:2001-02-18

The effect of low - intensity microwave radiation on cultured rat cortical cells
Liu Weiguo,Yang Xiaofeng,Goo Feng,et al.. The effect of low - intensity microwave radiation on cultured rat cortical cells[J]. Zhejiang Journal of Traumatic Surgery, 2001, 6(3): 141-142
Authors:Liu Weiguo  Yang Xiaofeng  Goo Feng  et al.
Affiliation:Liu Weiguo,Yang Xiaofeng,Goo Feng,et al. The Second Affiliated Hospital,College of Medical Sciences,Zhejiang University,Hangzhou 310009,China
Abstract:Objective To investigate the effect of low - intensity microwave radiation on cultured neonatal rat cerebellar corticalneurons. Methods During the experiment , the neuron were radiated with microwave ,which was frequency of 900MHz and power density of 0.025 mW/cm2,0.05mW/cm2,0. 1mW/cm2 for 4, 8. 12, 16, 20, 24h, and the cell viablity were assayed. Results It was found that the cell loss in the 0.05mW/cm2( 12h) and 0. lmW/cm2(8h) irradiated group were markedly higher than those in the control, which was positively related to the length of exposing time. However, there was no significance difference between the normal contral group and 0.025 mW/ cm2 irradiated group. Conclusion The results indicate that the longer and continual low - intensity microwave radiation can cause damage effect on neuron in vitro .
Keywords:low - intensity microwave  neuron culture  cell viablity
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