大鼠肝部分切除后再生过程中胰岛细胞的变化

本研究探讨大鼠肝部分切除65％和32％后1、2、3、7、14天期间,胰岛细胞数目和分泌活动的变化。取胰尾,用醛品红,Grimelius 法和 Hellerstrom—Hellman 法分别显示胰岛 B、A 和 D 细胞,计算上述三种岛细胞染色深度和分泌颗柱数量百分数,作为判断岛细胞分泌活动的指标。结果如下:1、A、B、D 三种岛细胞的数目均无明显改变,2、65％肝邵分切除后,胰岛细胞分泌活动的变化是:术后第1、2天,B 细胞分泌减弱,A 细胞稍强;术后第3天,B 细胞的分泌活动明显增强,A 细胞分泌继续增强,D 细胞分泌一直保持正常水平;术后第7天,B 细胞的分泌大为减弱,到术后第14天尚未恢复正常,在此期间,A 细胞分泌仍加强,D 细胞分泌也增强,3、32％肝部分切除后,岛细胞分泌活动的变化情况基本如上,只是变化的幅度较小和进程较快,其中以 B 细胞最明显。上述变化均与肝再生过程密切相伴。B细胞的变化似乎直接与肝再生相关,A 和 D 细胞可能起协同作用。

Histological Changes of Panereatic Islet Cells Following Partial Hepatetomy

The present study was designed to investigate the changes in num-ber and secretory activity of the islet cells following 65% and 32% partial hepatectomy in adult fe-male rats.Specimens of the panc-reatic tail were taken and stained with Aldehyde-fuchsin,Grimelius method and Hellerstrom-Hellman's method respectively in order to demonstrate B、 A and D cells The changes of secretory activity were judged by the stain intensity and number of secretory granules of different types of islet ceils. The results were as follows: 1.The number of B、A、 D cells of Langerhans islet did not show significant changes 2.After 1 or 2,days of 65% partial hepatectomy,the secretory activity of B cells declined and A ceils enhanced slightly.3days after the operation,the secretory activity of B cells increased appa-rantly,that of A cells enhanced continuously,that of D cells rem-ained at normal level.7 days after the operation,the secretory activity of B cells declined markedly, until 14 days,the secretory acti-vity did not recover to normal yet. During this period,the secretory activity of A ceils still increased, and that of D cells also raised 3.After 32% partial hepatecto-my,the changes in secretory activity of islet cells were basica-lly similar to that mentioned abo-ve,merely the changes were rel-atively mild,and its course was short.Among three types of islet cells,the changes of B cell we-re the most prominant. 4.The changes of islet ceils were closely related with the rege-nerating process of the liver.The changes of B cell seemed to be directly related to liver regenera-tion,A and D cell may coordin-ated with B cell.