| 重组Shh因子对海水浸泡肺微血管内皮细胞增殖和凋亡的影响研究 |
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| 引用本文： | 杨昱,钱桂生,王关嵩,邓朝霞,周长喜.重组Shh因子对海水浸泡肺微血管内皮细胞增殖和凋亡的影响研究[J].第三军医大学学报,2009,31(13). |
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| 作者姓名： | 杨昱 钱桂生 王关嵩 邓朝霞 周长喜 |
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| 作者单位： | 第三军医大学新桥医院全军呼吸内科研究所,全军呼吸病研究重点实验室,重庆,400038 |
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| 基金项目： | 全军医学科研计划科技攻关项目，国家自然科学基金 |
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| 摘 要： | 目的 观察不同剂量重组Shh(recombinant sonic hedgehog,rShh)因子对海水浸泡肺微血管内皮细胞(pulmonary microvascular endothelial cell,PMVEC)的促进增殖及抗凋亡作用.方法 组织块法原代培养大鼠PMVEC,建立大鼠PMVEC海水浸泡模型,加入不同剂量重组Shh因子,采用TUNEL法检测PMVEC凋亡率.采用2,3-苯基溴化四唑(MTT)染色计数法测定细胞增殖活性.结果 海水浸泡可导致PMVEC损伤,表现为PMVEC细胞增殖受到抑制以及凋亡发生率增加.重组Shh能降低海水所致PMVEC增殖抑制率和其凋亡率,在实验剂量范围内,其促增殖和抗凋亡作用呈剂量依赖性.结论 重组Shh可促进海水浸泡PMVEC增殖和抑制其凋亡.
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| 关 键 词： | 海水 肺微血管内皮细胞 急性肺损伤 |
Recombinant sonic hedgehog promotes proliferation and suppresses apoptosis of pulmonary microvascular endothelial cells exposed to seawater |
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| YANG Yu,QIAN Gui-sheng,WANG Guan-song,DENG Zhao-xia,ZHOU Chang-xi.Recombinant sonic hedgehog promotes proliferation and suppresses apoptosis of pulmonary microvascular endothelial cells exposed to seawater[J].Acta Academiae Medicinae Militaris Tertiae,2009,31(13). |
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| Authors: | YANG Yu QIAN Gui-sheng WANG Guan-song DENG Zhao-xia ZHOU Chang-xi |
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| Institution: | YANG Yu,QIAN Gui-sheng,WANG Guan-song,DENG Zhao-xia,ZHOU Chang-xi (Institute of Respiratory Diseases,Xinqiao Hospital,Third Military Medical University,Chongqing 400038,China) |
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| Abstract: | Objective To observe the effects of recombinant sonic hedgehog (rShh) on the proliferation and apoptosis of cultured pulmonary microvascular endothelial cells (PMVECs) after exposure to seawater. Methods After PMVECs were primarily isolated from SD rats and cultured, and then the cells were exposed to seawater (from the South China Sea) and treated with 50,100, 200 and 400 ng/ml rShh respectively for 24 h. Apoptotic rate of PMVECs was detected by TUNEL. The proliferation of PMVECs was examined by MTT assay.... |
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| Keywords: | Shh |
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