APP/PS1 转基因小鼠的甲状腺功能变化

 目的观察APP/PS1转基因小鼠甲状腺功能的动态变化并探讨AD与甲状腺功能的关系。方法6 月龄雌性APP/PS1
转基因鼠（ n=9）和野生型小鼠（ n=9）自由摄食和饮水，2 组小鼠分别于9，12，18 月龄时各取3 只处死，应用ELISA方法检测血
清TSH 和甲状腺激素（TT3、TT4、FT3、rT3）。结果（1）APP/PS1转基因小鼠与野生型小鼠相比血清TT3、FT3 水平均有降低，但
9 月龄时差异不显著（ P> 0.05），12 月龄时差异显著（ P< 0.05），18 月龄时差异更为显著（ P< 0.01）（2）APP/PS1转基因小鼠与
野生型小鼠相比血清TT4、rT3 均升高，但9月龄时差异不显著（ P> 0.05），12 月龄时差异显著（ P< 0.05），18 月龄时差异更加显
著（ P< 0.01）（3）各年龄段APP/PS1 转基因小鼠血清TSH 水平较野生型小鼠虽有所降低，但无统计学意义（ P> 0.05）。结论
APP/PS1 转基因小鼠在生长过程中伴随着甲状腺功能的改变，且逐渐加重。

Investigation on the Change of the Function of Thyroid Gland in APP/PS1 Transgenic Mice

Objective To investigate the change of the function of thyroid gland and the relation of Alzheimer’s disease （AD）to the
function of thyroid gland in APP/PS1 transgenic mice. Methods 6-month-old APP/PS1 transgenic and wild-type female mice（n=9/group）
were housed with food and tap water available ad libitum. Three mice of each group were sacrificed at the age of 9、12 and 18 months and
the serum were harvested to analyses the levels of TSH，TT3，TT4，FT3，rT3 with ELISA. Results （1）Compared with the wild-type mice，
the levels of TT3、FT3 in the serum of APP/PS1 transgenic mice decreased，which was not evident at the age of 9 months （ P> 0.05），but
was evident at the age of12 months（ P< 0.05），and more evident at the age of 18 month of age（ P< 0.01）.（2）Compared with the wild-
type mice，the levels of TT4、rT3 in the serum of APP/PS1 transgenic mice increased，which was not evident at the age of 9 months （ P>
0.05），but evident at the age of12 months（ P< 0.05），and more evident at the age of 18 month of age（ P< 0.01）.（3）The levels of TSH
showed no obvious difference between the two groups at the age of 9、12 and 18 months（ P> 0.05）. Conclusion With the development of
AD，there was significant and worsening changes of TT3、TT4、FT3、FT4、rT3 in the serum of APP/PS1 transgenic mice compared with the
wild-type mice.