人膀胱癌耐药细胞系BIU-87/A、BIU-87/V的建立及耐药分析

为研究人膀胱癌细胞株耐药机制，应用阿霉素（ＡＤＭ）、足叶乙甙（ＶＰ－１６）低浓度持续递增法，在体外持续培养１０个月，逐步诱导人膀胱癌细胞系ＢＩＵ－８７，获得具有多重抗药性的ＢＩＵ－８７／Ａ、ＢＩＵ－８７／Ｖ细胞。通过检测抗癌药物５０％抑制率剂量，ＢＩＵ－８７／Ａ、ＢＩＵ－８７／Ｖ细胞分别对ＡＤＭ、ＶＰ－１６的耐药程度较亲本细胞提高５７．４２倍、５．４４倍。两种耐药株显示了相似的耐药谱，均对ＡＤＭ、ＶＰ－１６、长春新碱和阿糖胞苷等药物产生耐药性，且随着培养时间延长，耐药性增强，但对丝裂霉素Ｃ、噻替哌和顺铂仍保持敏感性。结果表明ＢＩＵ－８７／Ａ、ＢＩＵ－８７／Ｖ细胞具有多重抗药性（ＭＤＲ）表型，为不同药物诱导人膀胱移行细胞癌ＭＤＲ机制和筛选抗药性逆转剂提供理想模型。

Establishment and Multidrug Resistance Analysis of BIU 87/A, BIU 87/V of Human Bladder Cancer Cell Lines

To study the resistance mechanism of human bladder cancer cell line, an adriamycin(ADM) resistance bladder cancer cell line, BIU 87/A and an etoposide (VP 16) resistance cell line, BIU 87/V were established by exposing the BIU 87 parent cells to a culture medium with a persistent increase of concentration of ADM or VP 16 respectively over a period of 10 months. The BIU 87/Acells became resistance to ADM 57.42 times , and the BIU 87/V cells to VP 16 5.44 times stronger than their parent cells at the concentration that caused 50 % inhibition of cell growth and exhibited cross resistance to ADM,VP 16,vincristine and alexan but not to mitomycin C, thiotepum and cisplatin. When BIU 87/A and BIU 87/V were cultured in the increasing dose drug continuously, the drug resistance property was more stronger. It was suggested that BIU 87/A and BIU 87/V had the phenotype of multidrug resistance with relative stability and could serve as an ideal model for the study of multidrug resistance in human bladder transitional cell carcinoma.