浅刺针法合通窍活血汤对脑梗死大鼠血浆TXB_2和6-酮-PGF1α水平的影响

目的研究浅刺针法合通窍活血汤对脑梗死大鼠血浆TXB2和6-酮-PGF1α水平的影响。方法将150只SD大鼠随机分为5组,Ⅰ、Ⅱ、Ⅲ、Ⅳ组均予脑梗死造模,Ⅴ组行假手术,之后Ⅰ组浅刺针法合通窍活血汤治疗,Ⅱ组予常规针法合通窍活血汤,Ⅲ组仅予通窍活血汤,Ⅳ组和Ⅴ组不予处理。各组于造模后2,7,14 d随机取10只大鼠采用放射免疫法测定大鼠血浆TXB2和6-酮-PGF1α的含量,并进行统计学处理。结果Ⅰ、Ⅱ、Ⅲ、Ⅳ组造模后血浆TXB2水平明显高于Ⅴ组(P均<0.01);与Ⅱ、Ⅲ、Ⅳ组比较,Ⅰ组TXB2水平下降更快(P均<0.01)。Ⅱ、Ⅲ、Ⅳ组6-酮-PGF1α显著低于Ⅴ组(P均<0.01);与Ⅱ、Ⅲ、Ⅳ组比较,Ⅰ组6-酮-PGF1α水平升高更快(P<0.01)。结论浅刺针法合通窍活血汤能更有效地降低TXB2水平,升高6-酮-PGF1α水平,使TXB2/6-酮-PGF1α比值趋向平衡,从而达到治疗脑梗死的目的。

Influence of the shallow thorn acupuncture combine with Tongqiao Huoxue soup to levels of blood plasma TXB2 and 6-keto-PGF1α on rats with cerebral infarctiOn

Objective It is to investigate the influence of the shallow thorn acupuncture combine with Tongqiao Huoxue soup to the levels of blood plasma TXB2 and 6-keto-PGF1α on rats with cerebral infarction.Methods 150 SD rats were randomly divided into 5 groups,Ⅰ,Ⅱ,Ⅲ and Ⅳ groups were made model of cerebral infarction,and Ⅴ group was gave sham operation.Ⅰgroup was treated combine the shallow thorn acupuncture and Tongqiao Huoxue soup,Ⅱgroup was treated combine the conventional acupuncture and Tongqiao Huoxue soup,Ⅲ group was treated with Tongqiao Huoxue soup,ⅣandⅤgroup did nothing. In the 2rd,7th and 14th day,each group randomly selected 10 rats and determined its levels of blood plasma TXB2 and 6-keto-PGF1α by radioimmunity method.Results In the 2rd,7th and 14th day,the levels of blood plasmas TXB2 in Ⅰ,Ⅱ,Ⅲ and Ⅳgroups were higher than Ⅴ group obviously(P all<0.01),compared with Ⅱ,Ⅲ and Ⅳ groups,the level of TXB2 were dropped quickly inⅠgroup (P all<0.01).The levels of 6-keto-PGF1α in Ⅱ,Ⅲ and Ⅳ groups were lower than Ⅴ group remarkably(P all<0.01),compared with Ⅱ,Ⅲ and Ⅳ group,the levels of 6-keto-PGF1α were rose quickly in Ⅰ group(P all<0.01).Conclusion The shallow thorn acupuncture combine with Tongqiao Huoxue soup could drop the level of plasma TXB2 and rise the level of plasma 6-keto-PGF1α,to be able effective improvement TXB2 /6-keto-PGF1α ratio trend balance,thus achieved treats goal of cerebral infarction.