天荔汤对糖尿病大鼠肾脏氧化应激的影响

目的研究糖尿病大鼠肾脏氧化应激反应,观察天荔汤对氧化应激的影响。方法将大鼠随机分为5组,即天荔汤高、低剂量组,西药对照组(Capotril HB419),病理组和正常对照组。用四氧嘧啶(Alloxan)诱导糖尿病大鼠模型,8周后检测大鼠血糖、肾脏肥大、肾组织匀浆超氧化物歧化酶(SOD)、丙二醛(MDA)、谷胱甘肽过氧化酶(GSH-Px)的活性。结果与正常组相比,病理组血糖、血脂、尿蛋白、肾重、体重等指标明显增高,肾匀浆MDA含量亦增加,而肾脏抗氧化酶的活性则降低,天荔汤显著降低肾组织匀浆SOD、MDA含量。结论糖尿病大鼠肾脏存在氧化应激反应,肾组织抗氧化酶缺乏。经天荔汤治疗后上述变化明显被逆转。

Effects of Tianli Decoction on Oxidative Stress in the Kidney of Diabetic Rat

Objective To investigate oxidative stress in the kidney of diabetic rat in order to tobserve the effect of Tianli Decoction(TLT) on diabetic nephropathy. Methods The rats were devided into five groups:TLD-High dose group,TLD-Low dose group,Captopril(Cap) HB419 group,pathologic control group,normal control group. Alloxan was used to induce the diabetic model of rats. After 8 weeks,blood glucose,blood lipid,urinary protein and the ratio of kidney weight to body weight in the rats of five groups were measured. The content of MDA and the renalintrinsic anti-oxidatantenzyme activities such as SOD and GSH-Px were evaluated. Results Comparing with normal group,the level of blood glucose,blood lipid,urinary protein and the ratio of kidney weight to body weight in diabetic group were significantly increased. The content of MDA in renalortex was increased,whereas the activities of anti-oxidatantenzyme were decreased. Conclusion Oxidativestress existed in the kidney of diabeticrats. The deficiency of intrinsic anti-oxidatantenzyme may contribute to the development of diabetic nephropathy. TLT can revers the obove changes.