白细胞介素8在大鼠脑缺血再灌注损伤中的变化

目的探讨白细胞介素8在大鼠脑缺血再灌注损伤中的变化.方法参照Zea Longa线栓法制作急性大鼠大脑中动脉缺血再灌注模型,将大鼠随机分为假手术组和脑缺血1h再灌注3、6、12、24及48h组,采用双抗体夹心间接酶联免疫吸附法检测大鼠血清和脑组织中白细胞介素8的浓度.结果血清中白细胞介素8含量于再灌注3h达最高(P＜0.01),随后缓慢下降,再灌注24h降至对照水平;脑组织中白细胞介素8含量于再灌注6h达高峰(P＜0.01),再灌注12h后下降,但再灌注6h与12h之间无显著性差异.结论白细胞介素8参与了脑缺血再灌注损伤的病理生理过程.

The Changes of Interleukin-8 Content after Cerebral Ischemia-Reperfusion Injury in Rats

Aim To study the change of the interleukin-8 (IL-8) content in blood and cerebrum after cerebral ischemia-reperfusion injury. Methods With Zea Longa's thread method, cerebral ischemia-reperfusion injury model of rats was established. The rats were randomly divided into the groups as sham operated control group and ischemia-reperfusion 3 h, 6 h, 12 h, 24 h and 48 h groups. The content of IL-8 in blood serum and brain tissue was detected by double antibody sandwich enzyme-linked immunosorbent assay. Results The content of IL-8 in blood serum was increased significantly at 3 h after reperfusion (P<0.01), and then went down. It reached the level of the control group at 24 h after reperfusion. The concentration of IL-8 in brain tissue was peaked at 6 h after reperfusion (P<0.01), went down after 12 h reperfusion. Moreover, there is no difference between the group of 6 h after reperfusion and 12 h after reperfusion (P>0.05). Conclusion IL-8 was involved in the pathophysiological course of cerebral ischemia-reperfusion injury.