β3整合素在生长因子促血管平滑肌细胞粘附和迁移中的作用

目的:探讨血小板衍生生长因子(PDGF)对β₃整合素表达的影响及β₃整合素在PDGF促血管平滑肌细胞(VSMC)粘附、迁移和增殖中的作用。方法:用抗β₃整合素胞外域抗体封闭VSMCβ₃整合素后,通过-TdR掺入、细胞粘附、细胞迁移分析及RT-PCR等方法,检测PDGF对β₃整合素表达及对VSMC粘附、迁移和增殖的影响。结果:阻断β₃整合素与细胞外基质(ECM)蛋白相互作用后,可在一定程度上抑制PDGF刺激的VSMC增殖,使-TdR掺入率降低39%;在β₃整合素抗体浓度为10mg/L时,PDGF引发的细胞粘附和迁移受到显著抑制(P＜0.05);PDGF作用于VSMC6h,β₃整合素基因表达活性达到峰值,比对照细胞高87%。结论:PDGF可显著诱导β₃整合素在VSMC中表达;β₃整合素与ECM蛋白相互作用在PDGF促VSMC粘附、迁移方面发挥重要作用。

Effects of β3 integrin on adhesion and migration of vascular smooth muscle cells induced by growth factor

AIM: To investigate the effect of platelet-derived growth factor (PDGF) on β₃ integrin gene expression and the role of β₃ integrin on adhesion, migration and proliferation of vascular smooth muscle cells (VSMC) induced by PDGF. METHODS: β₃ integxin gene expression was detected by RT-PCr. After β₃ integrin extracellular do-main was blocks, VSMC adhesio, migration and proliferation were measured by adhesion assay awound-culture model an ³H]-TSR incorporation respectively.RESULTS: After the interaction between β₃ integrin and extracellular matrix was blocked, VSMC proliferation was inhibited in some degree and the rate of ³H]-TdR incorporation into VSMC decreased 39%. The cell adhesion and migration were significantly inhibited when 10 mg/L anti-β₃ integrin antibody was added (P＜0.05). When VSMC were treated by PDGF for 6 hours, the expression of β₃ integrin gene was 87% higher than that of control. CONCLUSION: PDGF significantly induces expression of β₃ integrin gene in VSMC, and the interaction between β₃ integrin and ECM protein may play an important role in VSMC adhesion and migration.