p27kip1、p16蛋白和增殖细胞核抗原在鼻咽癌中的表达及其意义

目的 研究p27^kip1、p16蛋白及增殖细胞核抗原(PCNA)在鼻咽癌(NPC)组织中的表达,探讨它们之间的关系及其与．NPC生物学行为及预后的相关性。方法应用免疫组织化学EnVision两步法检测66例鼻咽非角化性癌(NKC)组织和25例鼻咽黏膜慢性炎症(NP)组织中p27^kip1、p16蛋白及PCNA表达水平。结果(1)．NKC组织中p27^kip1、p16阳性表达率分别为65％、68％;与NP组比较,差异有统计学意义(P＜0．05)。(2)＜、p16蛋白在NKC中的表达与NKC颅神经侵犯及治疗后5年生存率有关(P＜0．05),与临床分期、淋巴结转移无关(P＞0．05);PCNA表达与．NKC临床分期及治疗后5年生存率有关(P＜0．05),与淋巴结转移、颅神经侵犯无关(P＞0．05)。(3)p27^kip1、p16及PCNA阳性表达之间有相关性(P＜0．05)。结论检测p27^kip1、p16蛋白及PCNA有助于综合评估NKC的预后。

Expression and clinical significance of p27(kip1), p16 and proliferating cell nuclear antigen in nasopharyngeal carcinoma

OBJECTIVE: To evaluate the roles of p27(kip1), p16 gene protein and proliferating cell nuclear antigen expression in nasopharyngeal carcinoma (NPC). METHODS: The EnVision immunohistochemical method was used to detect the expression of p27(kip1), p16 gene protein and PCNA in 66 cases of non-keratinized carcinoma (NKC) and 25 cases of non-tumor nasopharyngeal tissue. RESULTS: (1) The positive expression rates of p27(kip1), p16 gene protein were 65%, 68% in NKC respectively. There were significant differences between NKC and non-tumor group (P < 0.05). (2) The expression of p27(kip1), p16 protein correlated with cranial nerve encroaching and the 5-year survival rates of the patients (P < 0.05), but had no significant correlation to lymph node metastases and clinical staging (P > 0.05). The expression of PCNA was related to clinical staging and to the patient's 5-year survival rates (P < 0.05), but not to lymph node metastases and cranial nerve encroaching (P > 0.05). (3) The positive expression of p27(kip1), p16 gene protein and PCNA were correlated. CONCLUSION: The results suggest that immunological labeling of p27(kip1), p16 gene protein and PCNA might be used to determine the prognosis of NKC.