二维电泳和质谱技术筛选寻常型银屑病皮损差异表达蛋白

目的:明确寻常型银屑病(psoriasis vulgaris,PV)皮损组织与正常人皮肤中的差异表达蛋白。方法:收集PV皮损组织及正常人皮肤组织。提取蛋白,进行二维凝胶电泳,选择在PV皮损组织中明显差异表达的蛋白点,进行质谱和生物信息学分析。结果:PV患者角质层和真皮层二维凝胶电泳鉴别蛋白总数分别为(1961±21)和(1928±49)个,确定差异表达蛋白30个,其中14个被鉴定分型:包括角质1型细胞骨架(K1C10、K1C14、K1C15、K1C16)、角质2型细胞骨架(K2C1),肌动蛋白(ARP3,ACTA,ACTBM),热休克蛋白(PHB,HSPβ1,CH60),中心体蛋白(CP135),膜联蛋白(ANXA4、ANXA5)。结论:差异表达蛋白可能在PV的病理形成中起重要作用,是潜在的PV诊断标记物。

Identification of differentially expressed proteins in psoriasis vulgaris using two-dimensional electro-phoresis and mass spectrometry

Objective：To identify the differentially expressed proteins in psoriasis vulgaris （PV） lesions and normal human skin tissue to propose potential pathological markers in psoriasis vulgaris. Methods：PV lesions and normal human skin tissues were collected and the proteins were extracted. And then, the proteins were resolved by immobilized pH gradient-based two-dimensional electrophoresis （ 2-DE） . The differentially expressed proteins were analyzed by matrix-assisted laser desorption /ionization time of flight mass spectrometry （MALDI-TOF-TOF-MS） and bioinformation. Results： Well-resolved 2-DE profiles of the epidermal and dermal layer were obtained,and average protein dots were （1969±21） and （1928±49） respectively;30 differentially expressed proteins were identified,14 of which were well characterized,including keratin type I,actin,heat shock protein,centrosomal protein and annexin. Conclusion：The differentially expressed proteins may play an important role in the pathogenesis of psoriasis and may be potential biomarkers in the diagnosis of PV.