血液疾患骨髓微血管密度的研究

为了探讨血液系统疾病骨髓血管新生的状态及意义，采用改良的乙二醇-甲基丙烯酸酯(GMA)塑料包埋骨髓病理切片和免疫组织化学染色检测贫血、急性髓细胞白血病(AML)、急性淋巴细胞白血病(ALL)、慢性髓细胞白血病(CML)患者的骨髓微血管密度(MVD)。结果显示，初诊的恶性血液病患者在治疗前骨髓MVD明显增加；急性白血病化疗完全缓解后骨髓MVD降至正常水平，未缓解者下降程度小，复发者再次升高至化疗前水平；贫血患者骨髓MVD也比对照组明显增高，但远不如急性白血病骨髓MVD增高的幅度大。结论：骨髓血管新生在急性白血病发生过程中有着重要的作用，抗血管新生治疗可能成为治疗白血病的新策略。

Study on Bone Marrow Microvascular Density in Hematological Diseases

To investigate the state and significance of bone marrow angiogenesis in hematological diseases, bone marrow microvascular density (BM MVD) in plastic embedded section was examined using acetone fixed bone marrow tissues embedded in glycol methacrylate (GMA) resin and by the method of immunohistochemistry. The results showed that bone marrow MVD increased greatly in newly diagnosed hematological malignancies before treatment. BM MVD in patients with acute leukemia decreased down to the normal range as the controls at the time of complete remission. In the non remission group, BM MVD decreased less, but when relapsed it increased again up to the same range as the newly diagnosed hematological malignancies, significant increase of BM MVD was found in patients with anemia, but in less degree than that in hematological malignancies. It is concluded that bone marrow angiogenesis plays a key role in the pathogenesis and development of hematological malignancy. Antiangiogenic therapy may be able to constitute a novel strategy for the treatment of hematological malignancies including leukemia.