硫酸氨基葡萄糖对佐剂关节炎大鼠镇痛作用及IL-6表达影响

目的研究盐硫酸氨基葡萄糖对佐剂性关节炎大鼠镇痛血液IL-6表达的影响,以探讨硫酸氨基葡萄糖在临床对类风湿关节炎的治疗作用及机制。方法30只SD大鼠分为三组。阳性对照组：通过给大鼠左后足跖部皮下注射完全弗氏佐剂（CFA）建立类风湿性关节炎的动物模型（AA）;治疗组：左后足跖部皮下注射完全弗氏佐剂（CFA）后第2d开始每日灌胃剂量2ml生理盐水含硫酸氨基葡萄糖100mg/kg连续28d;阴性对照组：左足跖部皮下注射0.1ml生理盐水。阴性对照组与阳性对照组每日灌胃2ml生理盐水。于造模后10d、12d、15d、17d、19d、21d、28d检测大鼠右足热痛阈。28d后处死大鼠检测血液IL-6表达情况。结果与模型组相比,治疗组大鼠右足热痛阈升高,血液IL-6表达降低。结论硫酸氨基葡萄糖对类风湿性关节炎的治疗具有一定的作用,其机制可能与降低IL-6等炎性因子有关。

Changes of the pain behavior and IL-6 after intragastric administration of aminoglucose in adjuvant arthritis rat

Objective To study the analgesia of glucosamine （GS） on rat adjuvant arthritis and changes of IL-6 with adjuvant arthritis rat. Methods 30 rats were randomly divided into 3 groups （10 in each group）： negative control group, positive control group, treatment group. positive control group： Intracutaneously injection of 0.1ml complete Freund＇s adjuvant （CFA） into the rats left hind limb set up an animal model of rheumatoid arthritis （AA）; treatment group： GS were taken by intragastric administration through 2th day post-injection CFA until 28th; negtive control group： Intracutaneously injection of 0.1ml saline into the rats left hind limb. Water were taken by intragastric administration in positive control group and negtive control group. The thermal threshold of the right hindpaws was examinated in the all three groups at 10d, 12d, 15d, 17d, 19d, 21d, 28d; The concentration of IL-6 was detected. Results The thermal threshold of the treatment group were mainly higher than the positive control group. The concentration of IL-6 was significantly decreased in the treatment group. Conclusion GS intaking had the positive influences for slow-down the progressing of rheumatoid arthritis（RA）.