生物源性和无机源性骨替代材料修复颅骨缺损的组织学变化

背景：目前有关生物源性和无机骨组织替代材料的研究时有报道,但是选择这两种材料对于颅骨缺损病理改变的骨增量和成骨效果的差异尚无定论。目的：比较兔颅骨缺损再生修复中应用生物源性和无机源性骨组织替代材料引导骨组织再生的能力。方法：建立成年新西兰兔颅顶骨3个直径8 mm洞型骨缺损模型,前方和后方洞型骨缺损区分别采用无机源性Bio-Oss骨粉与生物源性天博固齿骨粉填充并用胶原生物膜覆盖。中间洞型骨缺损区仅用胶原生物膜覆盖不做其他处理,作为对照组。结果与结论：术后12,16,24周通过Masson三色染色图像分析显示,无机源性骨粉组和生物源性骨粉组较空白对照组新生骨量明显增加,且生物源性骨粉组新生骨量明显多于无机源性骨粉组 (P ≤ 0.05)。可见无机源性与生物源性骨粉均可起到引导新骨再生修复骨缺损的作用,但生物源性骨粉修复骨缺损效果优于无机源性骨粉。

Histological changes of bio-derived and inorganic-derived bone tissue substitutes for repairing calvarial defects

BACKGROUND:At present, there are several reports about studies on bio-derived and inorganic-derived bone tissue substitutes. But the difference between the two bone materials in bone augmentation guided by calvarial defects and osteogenic effects is debatable.OBJECTIVE:To compare the ability of guiding new bone regeneration between the two bone materials during the repair of calvarial defects in rabbits.METHODS:A rabbit model of calvarial defects with three 8 mm diameter holes was established. The front and rear bone defects areas were filled separately with inorganic-derived and bio-derived bone materials. The middle bone defects areas were only covered with collagen membranes as a control.RESULTS AND CONCLUSION:At weeks 12, 16 and 24 after treatment, the Masson’s trichrome staining image showed that the new bone quantity of the two bone materials was obviously more than that of the blank control group at each time point; and the new bone quantity in the bio-derived bone materials group was obviously more than that of the inorganic-derived bone materials group at each time point (P ≤ 0.05). These results suggest that inorganic-derived and bio-derived bone materials can both guide new bone regeneration for repairing bone defects, but the effectiveness of bio-derived bone materials is superior to that of the inorganic-derived bone materials.