肝星状细胞活化增殖和凋亡及奥曲肽的影响

背景：肝星状细胞的活化增殖在肝硬化的发生发展中起关键作用,临床广泛应用的生长抑素衍生物奥曲肽有多种生物学效应,但它对肝星状细胞的活化增殖及凋亡有何影响尚不清楚。目的：观察奥曲肽对大鼠肝星状细胞增殖和凋亡的影响。方法：实验选用肝星状细胞株HSC-T6的传代细胞。MTT法检测不同浓度(1×10^-2,1×10^-3,1×10^-4,1×10^-5,1×10^-6,0 mmol/L)奥曲肽干预24,48,72 h对肝星状细胞增殖的影响;TUNEL凋亡检测试剂盒荧光显微镜方法检测不同浓度(0,1×10^-5,1×10^-2 mmol/L)奥曲肽干预24 h对肝星状细胞凋亡的影响。结果与结论：奥曲肽浓度越高、作用时间越长对培养的肝星状细胞增殖抑制越明显,呈现浓度和时间依赖性;奥曲肽对培养的肝星状细胞凋亡随奥曲肽浓度的增加而增加。结果可见奥曲肽对培养的肝星状细胞增殖抑制呈浓度 (0~10^-2 mmol/L)和时间(24,48,72 h)依赖性,并能诱发其凋亡。

Octreotide effects on the proliferation and apoptosis of hepatic stellate cells

BACKGROUND:The proliferation and apoptosis of hepatic stellate cells play an important role in the development of hepatocirrhosis. Octreotide that is a somatostatin derivative widely used in clinic has many biological effects, but the effect of octreotide on the proliferation and apoptosis of hepatic stellate cells is unclear.OBJECTIVE:To investigate the effect of octreotide on the proliferation and apoptosis of rat hepatic stellate cells.METHODS:The passage cells of hepatic stellate cells lines HSC-T6 were collected. The effect of different concentrations (1×10-², 1×10^-3, 1×10^-4, 1×10^-5, 1×10^-6, 0 mmol/L) of octreotide on the proliferation of hepatic stellate cells was detected with MTT assay after induced for 24, 48 and 72 hours. Apoptosis of hepatic stellate cells after induced by different concentration octreotide (1×10^-5, 1×10^-2 mmol/L) for 24 hours was evaluated by TUNEL staining.RESULTS AND CONCLUSION:The higher concentration and longer induction time of octreotide, the inhibitional effect of octreotide on the proliferation of hepatic stellate cells was more obvious (in a dose- and time-dependent manner). The apoptosis of hepatic stellate cells was obviously raised with the increased concentration of octreotide. The results showed that the octreotide inhibited the proliferation of hepatic stellate cells in a dose (0-1x10^-2 mmol/L)- and time (24, 48, 72 hours)-dependent manner and could induce the apoptosis of hepatic stellate cells.