四种细胞移植重建C57BL雌鼠免疫功能的对比

背景：据报道肌肉来源的细胞能重建造血功能，还需要进一步观察其他系细胞是否也能重建造血功能。 目的：对比不同细胞对C57BL雌鼠造血功能恢复的作用。 方法：摘眼球取绿色荧光蛋白转基因雄鼠外周血，溶血后制成白细胞，再取脾脏、肝脏制成脾细胞和肝细胞悬液，同时制备骨髓细胞。将C57BL雌鼠半致死量射线照射后，回输4种细胞，并在移植后18，39，53 d检测外周血绿色荧光蛋白阳性细胞。同时标记CD4-PE和CD8-PE，并在移植后100 d杀鼠取骨髓检测Y染色体阳性率。 结果与结论：移植后18，39，53 d均在C57BL雌鼠外周血检测到绿色荧光蛋白阳性细胞；C57BL雌鼠外周血绿色荧光蛋白阳性细胞百分比：回输脾细胞和骨髓细胞>回输肝细胞>回输外周血白细胞。同时在回输脾细胞和骨髓细胞的C57BL雌鼠外周血中检测到绿色荧光蛋白和CD4双阳性细胞、绿色荧光蛋白和CD8双阳性细胞，而回输肝细胞和外周血白细胞的C57BL雌鼠外周血中几乎未检测到双阳性细胞。移植后100 d检测到的Y染色体阳性率与外周血中绿色荧光蛋白阳性率呈正相关。结果说明绿色荧光蛋白转基因雄鼠的外周血白细胞、脾细胞、肝细胞和骨髓细胞均有重建造血功能的作用，其中脾细胞与骨髓细胞作用相似，作用大于肝细胞，肝细胞作用大于白细胞。

Four kinds of cells transplantation for reconstructing immune function of C57BL female mice

BACKGROUND:It is reported that muscle-derived cells can rebuild the hematopoietic function, whether the other cells can rebuild the hematopoietic function also needs further observation. OBJECTIVE:To compare the effect of different cells on the recovery of hematopoietic function of the C57BL female mice. METHODS:We removed the eyeballs of green fluorescent protein transgenic male mice in order to collect the peripheral blood. Then the bloods were hemolyzed to prepare white blood cells. And then, the spleen and the liver were obtained to prepare the spleen cells and liver cells suspension. The bone marrow cells were prepared. The C57BLfemale mice were irradiated by half-lethal dose and transfused with four kinds of cells. At 18, 39 and 53 days after transplantation, green fluorescence protein-positive cells in peripheral blood were detected. At the same time, the bloods were marked with CD4-PE and CD8-PE. At 100 days after transplantation, mice were sacrificed and their bone marrows were collected to detect Y chromosome-positive rate. RESULTS AND CONCLUSION:At 18, 39 and 53 days after transplantation, green fluorescence protein-positive cells were detected in peripheral blood of C57BL female mice; the percentage of green fluorescence protein-positive cells in the peripheral blood of C57BL female mice: transfused with spleen cells and marrow cells > transfused with liver cells > transfused with peripheral white blood cells. At the same time, the CD4 and green fluorescence protein double positive cells and CD8 and green fluorescence protein double positive cells were also detected in the peripheral blood of C57BL female mice after transfused with spleen cells and marrow cells, while the double positive cells were not detected in the peripheral blood of C57BL female mice after transfused with liver cells and peripheral white blood cells. At 100 days after transplantation, the Y chromosome-positive rate in bone marrow was positively correlated with the green fluorescence protein-positive rate in peripheral blood. The white blood cells, spleen cells, liver cells and effect marrow cells in the green fluorescent protein transgenic male mice can rebuild hematopoietic function, and the effect of spleen cells is similar to bone marrow cells but stronger than liver cells. The effects of liver cells are stronger than the white blood cells.