骨髓间充质干细胞在骨缺损修复中的应用

背景：在一定条件下骨髓间充质干细胞具有分化为成骨细胞、软骨细胞的特性,成为骨组织工程中研究最为深入的种子细胞。 目的：对骨髓间充质干细胞在骨缺损中的基础研究与临床应用进展作一综述。 方法：应用计算机检索CNKI和Pubmed数据库中1999年1月至2012年1月关于骨髓间充质干细胞应用于骨缺损治疗的文章,在标题和摘要中以“骨髓间充质干细胞,骨缺损”或“ Bone mesenchymal stem cells,bone defect”为检索词进行检索。选择文章内容与骨髓间充质干细胞治疗骨缺损有关者,同一领域文献则选择近期发表或发表在权威杂志文章。最终选择30篇文献进行综述。 结果与结论：骨髓间充质干细胞分离获取方法成熟,具有成骨细胞定向诱导分化能力,能有效地进行骨质缺损修复,尤其随着生物支架材料和基因工程技术的发展,骨髓间充质干细胞在骨缺损治疗中的应用必将更加广泛。

Application of bone marrow mesenchymal stem cells in the treatment of bone defects

BACKGROUND:Bone marrow mesenchymal stem cells have multilineage differentiation potential, which can be induced to differentiate into osteoblasts and chondrocytes under special conditions in vitro or in vivo. They are widely used for bone tissue engineering. OBJECTIVE:To review the basic research and clinical application of bone marrow mesenchymal stem cells for repair of bone defects. METHODS: The CNKI database and PubMed database (1999-01/2012-01) were used to search the related articles about the application of bone marrow mesenchymal stem cells for repair of bone defects. The key words are “bone marrow mesenchymal stem cells, bone defect”. Articles concerning bone marrow mesenchymal stem cells for the treatment of bone defects were included. For the articles that published recently or in the high-impact journals were preferred, and articles with repetitive contents were ruled out. Then 30 articles were suitable for further analysis. RESULTS AND CONCLUSION:Bone marrow mesechymal stem cells can be easily isolated from bone marrow. Bone marrow mesenchymal stem cells can be used to repair bone defects effectively because of their ability to differentiate into osteoblasts, especially with the development of biological scaffold materials and gene engineering technology.