脑源性神经营养因子促进低血糖幼鼠海马神经干细胞的定向分化

背景：有研究表明脑源性神经营养因子可以维持神经元的存活、影响神经元的迁移,在体外可以促进神经干细胞的存活和分化。 目的：探讨脑源性神经营养因子对低血糖幼鼠海马神经干细胞定向分化的作用。 方法：取新生1 d低血糖模型大鼠脑海马组织进行原代、传代及单细胞克隆培养。培养的细胞一部分进行神经干细胞鉴定,另一部分依据培养液中脑源性神经营养因子质量浓度的不同将单克隆细胞分为0,100,200 μg/L组,取第4代细胞进行诱导分化,行神经元特异性烯醇化酶免疫荧光染色,计数阳性细胞比例。 结果与结论：单克隆培养后3组细胞均呈巢蛋白阳性,诱导分化后细胞呈行神经元特异性烯醇化酶和胶质纤维酸性蛋白阳性;100,200 μg/L组神经干细胞生长较快,且分化为神经元特异性烯醇化酶阳性细胞比例较高(P < 0.05),但两组神经干细胞之间差异无显著性意义(P > 0.05)。提示脑源性神经营养因子促进低血糖幼鼠海马神经干细胞向神经元定向分化。

Brain-derived neurotrophic factor promotes the directional differentiation of hypoglycemia neonatal rat hippocampal neural stem cells

BACKGROUND:There is evidence that brain-derived neurotrophic factor can maintain neuronal survival, influence neuronal migration, and promote the survival and differentiation of neural stem cells in vitro. OBJECTIVE:To investigate the effects of brain-derived neurotrophic factor on the directional differentiation of hypoglycemia neonatal rat hippocampal neural stem cells. METHODS:Neonatal 1-day hypoglycemia rat hippocampal tissue was harvested and subjected to primary culture, subculture and cell monoclonal culture. Then cells were divided into two parts: one part was used for identification of neural stem cells, and the other part was divided into three groups as per different concentrations of brain-derived neurotrophic factors (0, 100, 200 μg/L). Passage 4 cells were used for induced differentiation. Neuron-specific enolase immunofluorescence staining was used for neuronal identification and positive cells were counted. RESULTS AND CONCLUSION:After monoclonal culture, three groups of cells were positive for nestin. After induced differentiation, cells were positive for neuron-specific enolase and glial fibrillary acidic protein staining. Neural stem cells in the 100 μg/L and 200 μg/L groups grew faster, and produced higher proportion of neuron-specific enolase-positive cells than those in the 0 μg/L group (P < 0.05). But there was no significant difference between 100 μg/L group and 200 μg/L group. These findings suggest that brain-derived neurotrophic factor promotes the directional differentiation of hypoglycemia neonatal rat hippocampal neural stem cells.