人脐血内皮祖细胞对脐动脉内膜损伤的修复作用

目的 体外分离培养人脐带静脉血内皮祖细胞(EPCs),观察EPCs对损伤脐动脉的修复作用.方法 采用磁珠分选法(MACS)从人脐血中分离培养EPCs,用流式细胞术、免疫细胞化学和免疫荧光检测进行鉴定;牵拉钳夹损伤法制备去内膜脐动脉段,与EPCs共孵育7 d后,通过病理切片、免疫组织化学和图像分析技术评价EPCs对动脉损伤的修复效果.结果 成功从人脐血中分离培养EPCs,流式细胞术分析结果 为培养7 d后CD133+细胞＞90%;CD34、vWF因子相关抗原免疫染色均为阳性.EPCs移植组新生内膜厚度(43.5±5.5)ìm显著低于对照组内膜厚度(90.7±12.7)ìm,(t=-28.88,P＜0.01);EPCs移植组的再内皮化程度(77.8±0.1)%明显高于对照组(52.2±0.1)%,(t=21.86,P＜0.01).结论 成功从人脐血中培养出EPCs,人脐血EPCs可修复内皮损伤血管.

Repair of arterial endothelium injury by umbilical cord blood transplantation of endothelial progenitor cells

Objective To culture endothelial progenitor ceils (EPCs) from human cord blood and observe the effect of EPCs on re-endothelialization of injured umbilical cord arterial endothelia. Methods Human cord blood-derived EPCs were selected by magnetic activated ceil-sorting system (MACS) and identiffed by flow cytometry,immuunocytochemistry and immunofluorescence staining. The umbilical cord arterial endothelium injury model was established. After co-culturo with EPCs for 7 days ,the samples were harvested and analyzed by elastic fibers stain blade to evaluate the repair of the injured umbilical cord arterial endothelia by EPCs. Results EPCs were isolated from the fresh human umbilical cord blood and cultured in vitro successfully. More then 90% of CD133 + cells were observed by using FACS after 7 days. The immuno-staining results of CD34 and vWF factor related antigens were positive. There was significant difference in the new intima thickness [(43.5 ±5.5) μm vs (90.7 ± 12.7) μm (t = -28.88,P＜0.01) ] and re-endothelialization [ ( 77.8 ± 0. 1 ) % vs ( 52.2 ± 0. 1 ) %, ( t = 21.86, P ＜ 0. O1 ) between EPCs-transplantation group and control group. Conclusion EPCs can contribute to the repair of the injuried endotbelia of vessels.