黄鼬干粉胶囊对再生障碍性贫血模型小鼠造血功能的影响

目的:观察黄鼬干粉胶囊对再生障碍性贫血(AA)模型小鼠造血功能的影响及作用机制。方法:采用X射线+环磷酰胺+氯霉素复合方法复制小鼠AA模型,观察黄鼬干粉胶囊对小鼠外周血象、骨髓有核细胞数(BMNC)、骨髓粒细胞-巨噬细胞集落生成单位(CFU-GM)、血清中可溶性细胞间黏附因子1(s ICAM-1)和骨髓组织病理学的影响。结果:黄鼬干粉胶囊能明显增加模型小鼠外周血中HGB、RBC、WBC和PLT数量,能增加骨髓BMNC数量,还能升高骨髓CFU-GM和降低血清s ICAM-1。与模型对照组比较,差异有统计学意义(P0.05)。骨髓组织病理学结果表明,黄鼬干粉能明显改善模型小鼠骨髓增生低下和造血灶减少,促进骨髓造血。结论:黄鼬干粉胶囊能改善AA模型小鼠造血功能,其作用机制可能与调节骨髓CFU-GM和血清s ICAM-1的平衡有关。

Influence of Yellow Weasel Dry Powder Capsules on Hemopoiesis of Mice Model with Aplastic Anemia

Objective: To observe the influence of yellow weasel dry powder capsules on hemopoiesis of mice model with aplastic anemia (AA) and the mechanism. Methods: Mice model with AA was prepared by X-ray, cy-clophosphamide and chloramghenicol, the influence of yellow weasel dry powder capsules on peripheral blood, bone marrow mononuclear cells (BMNC), colony forming unit-granulocyte and macrophage (CFU-GM), soluble intercel-lular adhesion molecules-1(sICAM-1) and the histopathology of bone marrow were observed. Results: Yellow weasel dry powder capsules could increase the numbers of HGB, RBC, WBC and PLT in peripheral blood of the mice obviously, increase the amount of BMNC, raise CFU-GM and decrease sICAM-1. Compared with model con-trol group, the difference showed statistical meaning (P<0.05). Histopathological results of bone marrow demon-strated that yellow weasel dry powder could notably improve hypofunction of bone marrow hyperplasia and the de-crease of hemopoietic focus of AA mice, and promote hemopoiesis of bone marrow. Conclusion: Yellow weasel dry powder capsules could effectively improve hemopoiesis of AA mice model, and its mechanism might be related to regulating the balance of CFU-GM and sICAM-1.