隐匿性乙型肝炎病毒感染的检测及临床特点分析

研究不明原因性肝炎患者中隐匿性乙型肝炎病毒（HBV）感染的巢式聚合酶链式反应（PCR）检测及HBV隐匿性感染的临床特点。不明原因性肝炎患者104例,收集临床资料及血清。HBV基因组S、X、C区分别设计2套巢式引物,比较扩增效率,挑选扩增效率高的引物巢式PCR扩增,检测出隐匿性HBV感染者。隐匿性HBV感染者与其它不明原因肝病患者易感因素、临床症状、体征、生化检查、影像学检查等方面比较分析。检测出隐匿性HBV感染13例。隐匿性HBV感染者较其它不明原因肝病患者年龄大,多为中老年患者,并且肝硬化比例明显高于后者。本组不明原因性肝炎患者中约有12.5%为隐匿性HBV感染。HBV隐匿性感染导致肝脏炎症,甚至肝硬化。

Detection and clinical characteristics analysis of occult hepatitis B virus infection

To investigate the detection of occult hepatitis B virus infection among liver diseases of unknown etiology using nested polymerase chain reaction method;to analyze the clinic characteristics of HBV occult infection.104 patients with liver disease of unknown etiology were enrolled in this study.To design and compare the efficacy of different sets of nested primers which were located at s gene,x gene an c gene of HBV genome respectively.Select the primers which were more efficiency to detect occult HBV infection.To analysis the clinical,biochemical features between HBV DNA positive cryptogenic hepatitis and HBV DNA negative cryptogenic hepatitis,13 cases were detected being infected with HBV.Patients of occult hepatitis infection were significantly older than patients in whom the HBV DNA were negative;meanwhile,the proportion of the decompensated cirrhosis in the former is more than that of in the latter.Occult HBV infections may account for a proportion of liver diseases of "unknown" etiology.Low serum level of HBV DNA may cause disease progression,even hepatocirrhosis.