HPLC-ELSD法测定银杏叶分散片中萜内酯的含量

目的建立银杏叶分散片中萜内酯的含量测定方法。方法采用蒸发光散射检测器，以ODS柱（150mm×4．6mm）为色谱柱，以正丙醇-四氢呋喃-水（1：15：84）为流动相。结果白果内酯在7．848～39．24μg、银杏内酯A在3．8～19．0μg、银杏内酯B在7．08-35．40μg、银杏内酯C在3．768～18．840μg范围内进样量的对数与峰面积的对数线性关系较好，平均回收率依次分别为98．39％（RSD=0．31％）、98．41％（RSD=0．20％）、97．07％（RSD=0．48％）、98．39％（RSD=1．61％）。结论本法准确，重现性好，适用于银杏叶分散片中萜内酯的含量测定。

Content Determination of Terpene Lactones by HPLC-ELSD in Ginkgo Leaves Dispersible Tablets

Objective To establish a method for determination of terpene lactones in Ginkgo Leaves Dispersible Tablets.Methods The evaporation light scattering detector(ELSD) and ODS column(150 mm×4.6 mm) were employed.The mobile phase consisted of propanol-tetrahydrofuran-water(1∶15∶84).Results The logarithm value of sample size had a good linear relationship with the logarithm value of peak area when the sample size of bilobalide and ginkgolide A,B and C was 7.848-39.240 μg,3.8-19.0 μg,7.08-35.40 μg,3.768-18.840 μg,respectively.The average recovery was 98.39%(RSD=0.31%),98.41%(RSD=0.20%),97.07%(RSD=0.48%),98.39%(RSD=1.61%),respectively.Conclusion This method is accurate with good repeatability for the content determination of terpene lactones in Ginkgo Leaves Dispersible Tablets.