益气复脉汤对脑缺血再灌注损伤大鼠ET及CGRP影响的实验研究

目的：观察益气复脉汤对脑缺血再灌注模型大鼠血浆内皮素（ET）和降钙素基因相关肽（CGRP）含量变化的影响，探讨大鼠脑缺血再灌注损伤的中医药治疗方法。方法：采用线栓法制备大鼠右侧大脑中动脉脑缺血再灌注损伤模型，分别用益气复脉汤高、低剂量组、尼莫地平水溶液予以治疗，然后采用放射免疫法检测血清ET和CGRP含量。结果：模型组脑缺血再灌注大鼠血浆ET含量明显增高，而CGRP下降，治疗后ET值比对照组下降明显（P〈0．01），CGRP值比对照组增高明显（P〈0．05）。结论：益气复脉汤通过降低ET和增高CGRP，能够对脑缺血再灌注损伤脑起保护作用，其疗效优于尼莫地平。

Empirical Study of Decoction of Tonifying Qi to Recover Pulse on the Content Change of ET and CGRP in Ischemical Reperfusion Injury Rat

Objective ： To observe the effect of decoction of tonifying qi to recover pulse on the content change of reperfusion model rat＇s blood plasma endothelin （ET） and calcitonin gene -related peptide （CGRP）,and investigate TCM therapeutic methods on ischemical reperfusion injury. Methods：Make the model of ischemical reper.fusion injury by ligating right arteria cerebri media of rat. Treat the model with high dose and low - dose of decoction of tonifying qi to recover pulse and Nimodipine water solution. Then detect ET and CGRP in serum by radio -immunity. Results：In model group ET raised up but CGRP dropped out. After treatment ET dropped out （P 〈 0. 05 ） but CGRP raised up markedly （ P 〈 0. 05 ）. Conclusion ： Decoction of tonifying qi to recover pulse can protect brain from ischemical reperfusion injury by cutting down ET and raising up CGRP, and its effect is better than Nimodipine.