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MYC和MUC1 mRNA联合检测在乳腺癌诊断中的应用
引用本文:李引钰. MYC和MUC1 mRNA联合检测在乳腺癌诊断中的应用[J]. 现代肿瘤医学, 2015, 0(22): 3240-3242. DOI: 10.3969/j.issn.1672-4992.2015.22.009
作者姓名:李引钰
作者单位:成都大学附属医院检验科,四川 成都 610000
摘    要:
目的:建立逆转录聚合酶链反应(RT-PCR)检测乳腺癌核内原癌基因(nuclear oncogene,MYC)和黏蛋白1(Mucin1,MUC1)基因表达水平,探讨其联合检测在乳腺癌诊断和治疗监测中的应用。方法:建立RT-PCR反应,并以β2-微球蛋白为内对照测定30例健康女性体检者、52例良性乳腺肿瘤和103例乳腺癌外周血中MYC和MUC1的表达量。结果:外周血中MYC和MUC1在乳腺癌组表达率显著高于良性乳腺疾病组及健康体检组,而良性乳腺疾病组和健康体检组的表达率无差异。MUC1和MYC联合检测的特异性显著高于单一基因。结论:RT-PCR联合检测外周血MUC1和MYC基因表达,可提高对乳腺癌诊断的特异性,具有标本来源容易、操作简便和价格便宜等优点,可有效辅助诊断乳腺癌。

关 键 词:逆转录聚合酶链反应  核内原癌基因  黏蛋白1  乳腺癌

Combined detection of MYC and MUC1 mRNA express in the diagnosis of breast cancer
Li Yinyu. Combined detection of MYC and MUC1 mRNA express in the diagnosis of breast cancer[J]. Journal of Modern Oncology, 2015, 0(22): 3240-3242. DOI: 10.3969/j.issn.1672-4992.2015.22.009
Authors:Li Yinyu
Affiliation:Clinical Laboratory in Affiliated Hospital of Chengdu University,Sichuan Chengdu 610000,China.
Abstract:
Objective: To evaluate combined detection of MYC and MUC1 gene in the diagnosis of breast cancer.Methods:We used RT-PCR to detect MYC and MUC1 mRNA express in clinical samples from 30 health women,52 patients with benign breast disease and 103 patients with breast cancer.β2-microglobin was used as internal control.Results:No significant difference was found in the positive rate of MYC and MUC1 between normal controls and benign breast disease group(P>0.05).The positive rate of MYC and MUC1 in breast cancer group were higher than those in normal controls and benign breast disease group(P<0.05).The specificity of combined detection of MYC and MUC1 gene express was higher than that of single gene detection(P<0.05).Conclusion:Combined detection of MYC and MUC1 in peripheral blood by RT-PCR can improve the specificity of diagnosis of breast cancer,with specimens from easy,simple and cheap,which can effectively assist the diagnosis of breast cancer.
Keywords:polymerase chain reaction  MYC  MUC1  breast cancer
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