大鼠椎骨错缝模型不同时间节点全血黏度变化的实验研究

目的：通过检测大鼠腰椎椎骨错缝模型不同时间点全血黏度的变化,研究石氏伤科气血理论及骨错缝、筋出槽学说,揭示慢性脊柱病损的病理生理学特征.方法：将36只350～450 g的SPF级雄性SD大鼠随机分为旋转固定组（RF组）、单纯固定组（SF组）和假手术组（Sham组）,每组12只.RF组和SF组大鼠腰椎L4-L6节段植入椎体外部连接固定装置,RF组大鼠使Ls棘突向右侧旋转,造成L5棘突与L4和Ls棘突的不共线;SF组大鼠单纯植入椎体外部连接固定装置不进行旋转.分别于固定后1、4、8和12周时,检测各组大鼠的全血黏度变化.结果：固定4、8周之后,RF组和SF组大鼠在高切（150/s）、中切（60/s）和低切变速率（10/s）下的全血黏度皆高于Sham组大鼠（P＜0.05）.固定1、12周后各组大鼠全血黏度比较差异无统计学意义（P＞0.05）.结论：椎体骨错缝、筋出槽可导致大鼠全血黏度升高,增加血脉瘀阻的程度,从而进一步诱发或加重脊柱病损的发生.

Experimental research on whole blood viscosity changes at different time points in rats model with lumbar vertebrae semidislocation

Objective：To investigate whole blood viscosity changes at different time points in rats model with lumbar vertebrae semidislocation,study Shi＇s theroy of qi and blood and ＂Gucuofeng and Jinchucao＂ （骨错缝 、筋出槽）,also reveal pathological physiology characteristics of spinal disorder.Methods：Thirty-six SPF male rats weighted 350 to 450 g were randomly divided into rotatory fixation group （RF group）,simple fixation group （SF group） and Sham group （Sham group）,12 rats in each group.Exterior vertebrae implanted through L4-L6 segments of lumbar vertebrae in RF and SF group were connected fixed device.In RF group,L5 spinous process were rotated to right,and caused L5 spinous process was non collinear with L4 and L6; In SF group.external fixed device were simple connected without rotation.At 1,4,8 and 12 weeks after fixation,whole blood viscosity changes were tested.Results：At 4 and 8 weeks after fixation,high （150/s）,medium （60/s） and lower （10/s） shear rate in RF and SF group were higher than that of Sham group （P＜0.05）.At 1 and 12 weeks,there was no sigificant differences among three groups in whole blood viscosity （P＞0.05）.Conclusion：＂Gucuofeng and Jinchucao＂（骨错缝、筋出槽） vertebrae could raise whole blood viscosity,increase degree of bloos stasis and induce or aggravate spinal disorder in further.