聚合酶链反应对结核杆菌的诊断应用

利用聚合酶链反应（PCR）对135例多类临床标本（痰、胸水及尿等）进行了检测，临床疑诊为结核病中105例，其中18例结核杆菌涂片阳性，PCR检测均为阳性；87例结核杆菌涂片阴性的标本，57例PCR检测阳性，总阳性率为71.4%，对照的30例非结核病的标本则无阳性，本研究结果表明，PCR在敏感性，特异性方面均优于传统的结核病诊断方法，它简捷、易行、直观、尤其适用于结核病的早期、快速诊断。

Application of Ploymerase Chain Reaction on the Diagnosis of Tuberculosis

PCR technique was used to detect the repetitive DNA sequences of Mycobacterium tu-berculosis in various clinical specimens of patients with tuberculosis. The amplification proto-col gave positve results in all of eighteen cases positive by smar and in 57 of 87 cases negativeby smear. The total positive rate detected by PCR wsa 71.4%and o in 30 controls.The re-sults shoued PCR was more sensitiv and specific than conventional methods in dete- cting My-cobacterium tuberculosis.It is simple,easy to perform and directly perceived by vision,espe-cially suitable in early and rapid diagnosis of tuberculosis.