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| RP-HPLC法测定盐酸青藤碱PLGA纳米粒的包封率及载药量 | |
| 引用本文： | 侯鹏伟,王东,范娇,于辉,彭海生,孙守丽. RP-HPLC法测定盐酸青藤碱PLGA纳米粒的包封率及载药量[J]. 中国医院用药评价与分析, 2010, 0(8): 719-720 |
| 作者姓名： | 侯鹏伟 王东 范娇 于辉 彭海生 孙守丽 |
| 作者单位： | [1]黑龙江省食品药品检验检测所,哈尔滨市150012 [2]哈尔滨医科大学大庆校区药学系,大庆市163319 [3]哈尔滨医科大学检验系,哈尔滨市150081 |
| 摘 要： | 目的：建立盐酸青藤碱PLGA纳米粒的包封率及载药量的测定方法。方法：SHIM-PACK VP-ODS柱,流动相为甲醇-10mol·L-1 NaH2PO4溶液（38∶62）,柱温为30℃,检测波长为262nm,流速1mL·min-1。结果：盐酸青藤碱在0.5～50μg·mL-1范围内线性关系良好,R2=0.9999,溶剂和辅料对其无明显干扰,平均回收率为95.31%,日内和日间RSD分别为1.31%和1.59%（n=5）。结论：该方法简便快速,准确可靠,可用于盐酸青藤碱PLGA纳米粒包封率及载药量的测定。 |
| 关 键 词： | RP-HPLC 盐酸青藤碱 PLGA纳米粒 包封率 载药量 |
RP-HPLC in Determination of the Entrapment Efficiency and Loading Amount of Sinomenine Hydrochloride in PLGA Nanoparticles |
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| HOU Peng-wei,WANG Dong,FAN Jiao,YU Hui,PENG Hai-sheng,SUN Shou-li. RP-HPLC in Determination of the Entrapment Efficiency and Loading Amount of Sinomenine Hydrochloride in PLGA Nanoparticles[J]. Evaluation and Analysis of Drug-Use in Hospital of China, 2010, 0(8): 719-720 | |
| Authors: | HOU Peng-wei WANG Dong FAN Jiao YU Hui PENG Hai-sheng SUN Shou-li |
| Affiliation: | 1.Heilongjiang Institute of Food and Drug Control,Harbin 150012,China; 2.Dept.of Pharmacy, Daqing Campus of Harbin Medical University, Daqing 163319, China; 3.Dept.of Clinical Laboratories,Harbin Medical University,Harbin 150081,China) |
| Abstract: | OBJECTIVE：To establish a RP-HPLC method to determine the entrapment efficiency and loading amount of sinomenine hydrochloride loaded by PLGA nanoparticles.METHODS：The determination was performed on SHIM-PACK VP-ODS column with a column temperature of 30℃ .A mobile phase consisted of methnol-10 mol· L-1NaH2PO4（38∶ 62） at a flow rate of 1 ml·min-1 with a detection wavelength of 262 nm.RESULTS：The linear range of sinomenine hydrochloride was 0.5-50 μg·mL-1（ r=0.999 9） and the results were free from interference of solvent and excipients.The average recovery was 95.31% ,and the intra-day and inter-day RSDs were 1.31% and 1.59% ,respectively.CONCLUSION：This method is simple,rapid,accurate and reliable,and it is suitable for determining the entrapment efficiency and loading amount of sinomenine hydrochloride in PLGA nanoparticles. |
| Keywords: | RP-HPLC Sinomenine hydrochloride PLGA nanoparticles Entrapment efficiency Drug loading amount |
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