HPV16E7抗原HLA-A2限制性CTL表位预测及其合成

目的：预测、合成、纯化及鉴定人乳头瘤病毒E7抗原的HLA－A2限制性细胞毒性T淋巴细胞表位,为表位抗原特异性鉴定和临床研究提供靶肽。方法：采用超模体、多项式和量化模体方案相结合的方法,对靶抗原HPV16E7的HLA－A2限制性细胞毒性T淋巴细胞（Cytotoxic T lymphocyte,CTL)表位进行预测,并运用固相合成法合成多肽,经RP－HPLC纯化及纯度分析,用质谱进行定性鉴定。结果：分别预测出了16个、10个和3个九肽表位,确定其中5个九肽为候选合成表位,各合成肽的纯度都在95％以上。经质谱分析,各肽的分子量测定值与理论值相符。结论：超模体、多项式和量化模体方案联合应用可提高预测效率,避免了在研究E7抗原表位时盲目合成多肽;所合成的多肽为高纯度靶肽,可用于后续实验研究。

Prediction and synthesis of HLA-A2-restricted CTL epitopes derived from human papilloma virus E7 antigen

Objective To predict the HLA A2 restricted CTL epitopes of human papilloma virus E7 antigen, and to synthesize, purify and identify the predic ted HLA A2 restricted CTL epitope candidates from HPV16E7 for the antigenicity identification and clinical experiment.Methods The CTL epitope s of E7 antigen were predicted by HLA A2 binding peptide supermotif prediction method combined with the polynomial and quantitative motif method. The peptides were synthesized with solid phase strategies, purified with reverse phase HPLC and identified with mass spectrometry. Their purities were also determined.Results Sixteen, ten and three CTL epitopes were respectively predi cted, among which 5 peptides were the candidates to be synthesized. All peptides were beyond 95% in purity and the measured values of molecular weight conformed to their theoretical values.Conclusions The combination of sup e rmotif with the polynomial and quantitative motif method can improve the predict ion efficiency and avoid synthesizing peptides aimlessly. The synthesized peptid es are the target peptides with high purity and are useful in further studies. [