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| 端粒酶催化亚基基因表达激活人胚肺成纤维细胞端粒酶活性 | |
| 引用本文： | 郑晓飞,朱捷,杨义军,江红,吕星,孙志贤. 端粒酶催化亚基基因表达激活人胚肺成纤维细胞端粒酶活性[J]. 军事医学科学院院刊, 2002, 26(2): 96-98 |
| 作者姓名： | 郑晓飞 朱捷 杨义军 江红 吕星 孙志贤 |
| 作者单位： | 1. 军事医学科学院放射医学研究所,北京,1008502. 2. 沈阳军区军事医学研究所,沈阳,110031 |
| 基金项目： | 国家重点基础研究规划资助项目 (G2 0 0 0 0 5 70 0 1)，国家自然科学基金资助项目 (3 0 0 70 85 9) |
| 摘 要： | 目的 :通过端粒酶催化亚基基因转入人胚肺成纤维细胞 ,激活人胚肺成纤维细胞端粒酶活性 ,探讨其在延缓细胞衰老中的作用。方法 :用PCI_hTRT质粒转染人胚肺成纤维细胞 ,G4 18筛选获得稳定表达端粒酶活性的人胚肺成纤维细胞 ,采用RT_PCR和TRAP法分别测定端粒酶催化亚基mRNA和端粒酶活性表达情况。结果 :获得了表达端粒酶活性的人胚肺成纤维细胞 ,其寿限长于正常人胚肺成纤维细胞。结论 :端粒酶催化亚基异位表达可以激活人胚肺成纤维细胞的端粒酶活性 ,并延长人胚肺成纤维细胞的寿命 |
| 关 键 词： | 端粒酶 成纤维细胞 细胞衰老 基因表达 |
| 文章编号： | 1000-5501(2002)02-0096-03 |
| 修稿时间： | 2001-12-07 |
Telomerase activation in human fibroblasts by expressing the catalytic component of human telomerase |
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| Abstract: | Objective:To investigate the role of telomerase activation in aging by maintaining cultures of hTRT_transduced human fetal lung fibroblasts. Methods:Following delivery of the hTRT gene to human fetal lung fibroblasts using a PCI_hTRT vector, cell clones that expressed functional telomerase activity were screened by G418. Telomerase activity, hTR RNA and hTRT mRNA in normal fibroblasts and hTRT_transduced human fetal lung fibroblasts were investigated.Results: Human fetal lung fibroblasts clones with positive telomerase were obtained, and the cellular life span of hTRT_transduced human fetal lung fibroblasts was extended.Conclusions:Ectopic expression of telomerase (hTRT) in human fetal lung fibroblasts has been shown to restore telomerase activity and to extend cellular life span. |
| Keywords: | telomerase fibroblasts cell aging gene expression |
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