影响冻融胚胎种植潜能的相关因素分析

目的:探讨冻融胚胎移植(FET)周期中影响胚胎种植潜能的相关因素。方法:回顾性分析165例进行冻融胚胎并实施单胚胎移植(SET)的患者,按患者年龄、冷冻前胚胎质量、冷冻方式、复苏后卵裂球存活状态、复苏后是否进行辅助孵化分组,分析不同分组各组间的胚胎种植能力差异。结果:年龄≥36岁组的胚胎种植率(17.4%)低于年龄<36岁组(26.1%),但两者比较无统计学差异;冷冻前胚胎质量评分≥20组的胚胎种植潜能优于胚胎质量评分<20组(29.4%vs13.0%,P<0.05);玻璃化快速冷冻法和程序慢速冷冻法对胚胎种植能力的影响无明显差异(25.6%vs24.6%,P>0.05);复苏后卵裂球完整存活的胚胎种植率为29.8%,优于复苏后卵裂球部分损伤者(13.7%)(P<0.05);激光辅助孵化对胚胎种植能力无明显影响(21.4%vs25.2%,P>0.05)。结论:冷冻前胚胎质量及冷冻后胚胎存活状态是影响冻融胚胎种植潜能的重要因素。

Analysis of Correlated Influencing Factors in Frozen-thawed Embryo Implantation Potential

Objective： To explore the impact factors on frozen-thawed embryo implantation （FET） potential. Methods： A retrospective analysis was performed on 165 FET cycles, in which one embryo was transferred. Patients were divided into different group according to the age, pre-freeze embryo quality, cryopreservation technique, post-thaw blastmere survival status, assisted hatching or not, respectively. The implantation rates were compared among groups. Results： The implantation rate was 17.4% in women older than 36 years old and 26.1% in women younger than 36 years old, while the difference was not significant （P〉0.05）. The implantation rate of embryo scored ≥ 20 was significantly higher than that of embryo scored less than 20 （29.4% vs 13.0%, P〈0.05）. Vitrification and slow-freezing technique had no significant difference in influencing the embryo implantation potential （25.6% vs 24.6%, P〉0.05）. Transfer with embryo characterized by fully intact achieved higher implantation rate （29.8%） as compared with embryo characterized by partially damaged （13.7%）（P〈0.05）. Whether assisted hatching was performed or not, it won＇t influence the embryo implantation potential （21.4% vs 25.2%, P〉0.05）. Conclusion： The pre-freeze embryo quality and post-thaw blastmere survival status are the most important factors which influence the embryo implantation potential.