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| 赖型钩端螺旋体基因文库的构建及重组质粒pDC38的初步研究 | |
| 引用本文： | 陈庄,戴保民,张义正,李胜富. 赖型钩端螺旋体基因文库的构建及重组质粒pDC38的初步研究[J]. 四川大学学报(医学版), 1995, 0(4) |
| 作者姓名： | 陈庄 戴保民 张义正 李胜富 |
| 作者单位： | 四川联合大学生物工程系分子生物学研究室 |
| 摘 要： | 应用质粒载体ｐＵＣ１８购建了黄疸出血群赖型钧体的基因文库，重组率８６％，重组子约１２０００个。以ＯｍｐＬ１基因片断作探针从该基因文库中筛出１０个阳性克隆，其中重组质粒ｐＤＣ３８与７型８株致病钩体（黄疸出血群赖型、大型、致热型、秋季型、澳大利亚型、波摩那型、流感伤寒群临海型）ＤＮＡ杂交有杂交信号；与非致病的双曲钩体、细丝体，大肠杆菌和２型问号钩体（爪哇型、拜伦型）ＤＮＡ无杂交信号。 |
| 关 键 词： | 钩端螺旋体，基因文库，重组质粒 |
Construction of Genomic Library of L. interrogans Serovar lai and Preliminary Study of Recombinant Plasmid pDC38 |
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| Chen Zhuang, Dai Baomin, Zhang Yizheng, Li Shengfu. Construction of Genomic Library of L. interrogans Serovar lai and Preliminary Study of Recombinant Plasmid pDC38[J]. Journal of Sichuan University. Medical science edition, 1995, 0(4) | |
| Authors: | Chen Zhuang Dai Baomin Zhang Yizheng Li Shengfu |
| Abstract: | A genomic library.consisting of ap-proximate 12000 recombinants,has been constructedfor the Leplospira inlerrogans serovar lai in E, coli.using pUC18 as the vector. Hybridization analysiswith the DNA fragment contalning OmpL1 gene wasperformed and 10 positive clones were screened fromthe genomic library. One of the positive clones,desig-nated pDC3 8,showed hybridization signal with theDNA of 7 serovars 8 strains of pathogenic leptospires,but not with the DNA of nonpathogenic leptospires(L,biflexa).Leptoanla illini,E. coli.,and 2strains of L, interongans. |
| Keywords: | Leptospires Genomic library Recombinant plasmid |
| 本文献已被 CNKI 等数据库收录！ | |