江西省流行性乙型脑炎病毒的分离与鉴定

目的调查江西省的虫媒病毒分布状况,在当地采集蚊虫标本进行病毒分离与鉴定。方法 2008-2009年夏季在江西省共选择8个标本采集点,使用诱蚊灯采集蚊虫标本,通过组织培养法分离病毒,并对病毒分离物进行血清学和分子生物学鉴定;利用生物信息学技术对新分离病毒的序列进行分析,完成同源性和系统发生分析。结果 2008年采集到3属3种共计11916只蚊虫标本;2009年采集到4属5种共计5905只蚊虫标本;共分离到4株病毒,经鉴定均为流行性乙型脑炎(乙脑)病毒;新分离乙脑病毒与基因Ⅰ型乙脑病毒的同源性最高,系统进化结果显示,4株乙脑病毒均属于基因Ⅰ型。结论在江西省分离到基因Ⅰ型乙脑病毒,与上海市和浙江省分离株进化关系较近。

Isolation and identification of Japanese encephalitis virus in Jiangxi province,China

Objective To investigate the distribution profiles of arboviruses in Jiangxi province,China.Methods Mosquito samples were collected using mosquito lamps at 8 sites in Jiangxi in the summers of 2008 and 2009 and then used for virus isolation by tissue culture.The obtained virus isolates were identified by serological and molecular biological methods.Homology and phylogenetic analysis was performed on the sequences of the isolates by bioinformatics.Results A total of 11 916 mosquitoes(3 species,3 genera) were collected in 2008,and 5905 mosquitoes(5 species,4 genera) were collected in 2009.Four virus strains were isolated from the mosquitoes and were all identified as Japanese encephalitis virus(JEV).All the JEV strains belong to genotype Ⅰ,according to the homology and phylogenetic analysis.Conclusion Genotype Ⅰ JEV strains were isolated from mosquitoes collected in Jiangxi province,China.And they have a close phylogenetic association with JEV strains from Shanghai and Zhejiang province,China.