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| MALDI质谱成像技术在狂犬病病毒小鼠组织内定位研究中的应用 | |
| 引用本文： | 徐静,杨松涛,万家余,张守峰,许娜,李楠,许琴,刘文森. MALDI质谱成像技术在狂犬病病毒小鼠组织内定位研究中的应用[J]. 中国免疫学杂志, 2012, 28(6): 540-543 |
| 作者姓名： | 徐静 杨松涛 万家余 张守峰 许娜 李楠 许琴 刘文森 |
| 作者单位： | 1. 军事医学科学院军事兽医研究所,长春,130122;吉林省人兽共患病预防与控制重点实验室,长春130122 2. 吉林医药学院,长春,130122 |
| 基金项目： | 国家农业公益性行业科研专项(200803014) |
| 摘 要： | 目的:建立基于MALDI质谱的狂犬病病毒小鼠脑组织内质谱成像实验方法,寻找狂犬病病毒脑组织内定位标记物。方法:制备冰冻切片,进行组织上原位酶解及基质覆盖,利用MALDI质谱扫描成像,以及FlexImaging 2.1软件分析,对差异肽段进行二级质谱鉴定。结果:初步鉴定出了四段狂犬病病毒肽段,可作为组织内狂犬病病毒定位标志物。结论:为进一步提高狂犬病病毒组织内定位的精确度奠定了研究基础。 |
| 关 键 词： | MALDI 狂犬病 病毒 分子成像 |
Analysis of rabies virus location in mouse brain tissue by MALDI imaging mass spectrometry |
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| XU Jing , YANG Song-Tao , WAN Jia-Yu , ZHANG Shou-Feng , XU Na , LI Nan , XU Qin , LIU Wen-Sen. Analysis of rabies virus location in mouse brain tissue by MALDI imaging mass spectrometry[J]. Chinese Journal of Immunology, 2012, 28(6): 540-543 | |
| Authors: | XU Jing YANG Song-Tao WAN Jia-Yu ZHANG Shou-Feng XU Na LI Nan XU Qin LIU Wen-Sen |
| Affiliation: | .Institute of Military Veterinary,Academy of Military Medical Sciences,Key Laboratory of Jilin Province for Zoonosis Prevention and Control Jilin Province,People’s Republic of China,Changchun 130122,China |
| Abstract: | Objective:To establish the imaging mass spectrometry based on MALDI-TOF-MS for analysis of rabies virus in mouse brain,and to locate markers in intela.Methods:Frozen sections were prepared,in situ trypsin digestion,matrix deposition and MALDI MS were performed,the data was analyzed by FlexImaging 2.1 software and the specific peptieds were identified by MALDI MS/MS.Results:Four peptides from rabies virus were identified on tissue,which could be acted as markers for location.Conclusion:This method can be a help for improving the accuracy rating of rabies virus location on tissue. |
| Keywords: | MALDI Rabies Virus Imaging |
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