快速荧光生长指示管检测法对各种抗酸分支杆菌培养的研究

目的　探讨快速荧光生长指示管培养法对各种抗酸分支杆菌菌株培养的适应性。方法 应用本室研制的快速荧光生长指示管(以下简称RFGIT),定量接种各种抗酸分支杆菌标准菌株,并与改良罗氏培养管(LJ管)和BD公司MGIT快速培养管进行配对比较;用结核分支杆菌H37Rv株梯度稀释,分别接种RFGIT、MGIT和LJ管,记录出现阳性结果时间。结果 RFGIT对19种标准菌株培养阳性检出时间,平均为4.68±2.48d;MGIT管培养为5.39±2.89d。有8种菌株RFGIT检出时间显著早于MGIT管。接种不同浓度H₃₇Rv,在3种培养管配对比较,RFGIT阳性检出时间较MGIT管提前1～5d,平均提前3.2d;较改良罗氏法提前11～31d,平均提前19.1d。结论 RFGIT对各标准菌株及不同浓度H₃₇Rv培养阳性的检出时间均明显早于改良罗氏培养管;部分菌株明显早于MGIT培养管。结果表明RFGIT是一种有效、安全、经济和快速的培养法。

The study on rapid culture detection in the standard strains of acid-fast mycobacterium using fluorescent growth indicator tube

Objective To approach the adaptability of the culture system of a rapid fluorescent growth indicator tube,using various strains of acid-fast mycobacterium.Methods The standard strains of all species were puantitatively inoculated in the rapid fluorescent growth indicator tube(RFGIT),which has been developed by our lab,and pair matching compared with Mycobacterial Growth Indicator Tube system(MGIT,Becton Dickinson,MD)and Lowenstein-Jensen(L-J)solid media.The gradient dilutions of Mycobacterium tuberculosis H₃₇ Rv were inoculated both RFGIT and MGIT or L-J media in pairs,the Results were recorded and compared.Results The average detection time of 19 species standard culture is 4.68±2.48 days for RFGIT and 5.39±2.89 days for MGIT.The detected times of eight strains in RFGIT are significantly early than MGIT.The different dilutions of H₃₇ Rv assay in RFGIT shows a earlier detecting 1～5 days than MGIT,average 3.2 days, and 11～31 days early than L-J media,average 19.1 days. Conclusion The detecting time of RFGIT are earlier than L-J media in all standard strains and different dilutions of H₃₇ Rv.RFGIT appears more early than MGIT in some standard strains. Our Results showed RFGIT is a useful,safe,economical and rapid culture system.