成骨细胞中NO及c-fos对流体剪切力的响应

目的：研究NO及c—fos对不同流体剪切力作用时间的生理响应。方法：分离新生SD大鼠颅盖骨中的成骨细胞并分成三组，分别使用含10％FBS、0．3mmol／L L—NMMA和0．1mmol／L SNP的DMEM培养液预处理24h，加载大小为1．2Pa的剪切力。每组在加载剪切力0min、10min、15min、30min、60min后分别测试c—fos mRNA、NOS的表达。结果：c—fos mRNA表达在水平剪切力加载15min时明显增高（P〈0．05），在使用L—NMMA后明显降低（P〈0．05），而使用SNP后明显升高（P〈0．05）。结论：c—fos和NO参与了力学刺激引发细胞响应的过程，特别是在外界信号刺激引起的信息传递级联反应中起重要的偶联作用。

Nitric oxide regulates c-fos expression in osteoblastic cells induced by wall-shear

Objective: To observe regulation of nitric oxide on c-fos expression in osteoblastic cells in response to changes in wall-shear stress in vitro.Methods: Isolated and purified osteoblastic cells from the calvaria of newborn SD rats were cultured and passaged.The third generation cells,pre-treated with 10% FBS DMEM,0.3 mmol/L L-NMMA DMEM and 0.1 mmol/L SNP DMEM separately,were subjected to wall-shear stress of 1.2 Pa.Gene expression of the c-fos and NOS activity were studied before(0 min) and 10 min,15 min,30 min,60 min after treated with wall-shear stress.Results: The expression of c-fos mRNA was increased transiently after application of 1.2 Pa wall-shear stress in osteoblastic cells and peaked at 15 min.The expression of c-fos mRNA was decreased after pre-application with L-NMMA and increased after use of SNP.Conclusion: Changes in the osteoblastic cells mechanical environment may cause a dramatic induction of NO and c-fos expression.