Morphological variability, lectin binding and Na+,K+-activated adenosine triphosphatase activity of isolated Müller (glial) cells from the rabbit retina |
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| Authors: | A Reichenbach D Dettmer G Brückner M Neumann G Birkenmeyer |
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| Affiliation: | 1. Carl Ludwig Institute for Physiology, Department of Neurochemistry, Karl Marx University, Leipzig G.D.R.;2. Institute for Physiological Chemistry, Department of Neurochemistry, Karl Marx University, Leipzig G.D.R.;3. Paul Flechsig Institute for Brain Research, Department of Neurochemistry, Karl Marx University, Leipzig G.D.R. |
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| Abstract: | Rabbit retinal Müller cells were isolated by means of papaine and mechanical dissociation. These cells were shown to have a well preserved morphology and to preserve viability for many hours. Intense wheat germ agglutinin binding occurs on the photoreceptor side of Müller cells, especially in the microvillous region. Rabbit retinal Müller cells have a Na+,K+-activated adenosine triphosphatase activity in the same order of magnitude as brain astroglial cells. |
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| Keywords: | rabbit retina Müller cells papaine dissociation wheat germ agglutinin binding Author for reprint requests at: Carl-Ludwig-Institute for Physiology, Karl Marx University, Liebigstrasse 27, DDR-7010, Leipzig, G.D.R. |
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